RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean (Glycine max L. Merr.)
RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean (Glycine max L. Merr.)
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RNA-seq 分析揭示乙烯介导的大豆 (Glycine max L. Merr.) 生殖器官发育和脱落
DOI：
10.1007/s11105-012-0533-4
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发表时间：
2013-06-01
影响因子：
2.1
通讯作者：
Liu, Qiang
中科院分区：
文献类型：
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作者：
Cheng, Yun-Qing;Liu, Jian-Feng;Liu, Qiang
大豆（Glycine max L. Merr.）发育中生殖器官的高度脱落会导致严重的产量损失。乙烯在加速器官脱落方面的作用已得到充分证明。然而，人们对乙烯影响大豆脱落的调控机制知之甚少。乙烯合成抑制剂 [0.5 mM 硫代硫酸银 (STS)]、乙烯释放化合物 [400 mg/L 乙烯利 (ETH)] 和水施用两次，两次施用之间间隔 5 天。经过 STS 处理的植物会产生更多的花和豆荚，从而使种子产量增加 55.6%。 ETH处理显着提高了花和荚果的脱落率（P < 0.05）。构建了三个数字基因表达库（STS-、ETH-和对照库）。使用 Illumina 对三个文库进行测序来鉴定 9.7 至 1050 万个 unigene。在不同基因表达谱和特定代谢物组（例如植物激素生物合成和信号转导；淀粉和蔗糖代谢；以及次生代谢）之间观察到强相关性，表明这些代谢途径在乙烯调节过程中的重要性。鉴定出潜在的乙烯靶基因，如1-氨基环丙烷-1-羧酸酯（ACC）氧化酶、2C蛋白磷酸酶（PP2Cs）、MAT1、乙酰转移酶、双向糖转运蛋白SWEET1-like等。这些结果表明，乙烯通过直接转录调节参与与花器官发育和脱落相关的代谢和调节过程的基因来调节大豆中的花和豆荚脱落。因此，这些发现进一步阐明了乙烯在生殖器官发育和脱落的转录调控中的关键作用。
The high level of abscission of developing reproductive organs in soybean (Glycine max L. Merr.) causes severe yield loss. The role of ethylene in hastening organ abscission has been well documented. However, little is known about the regulatory mechanism by which ethylene influences abscission in soybean. Ethylene synthesis inhibitor [0.5 mM silver thiosulfate (STS)], ethylene-releasing compound [400 mg/L ethephon (ETH)], and water were applied twice with a 5-day interval between the applications. The STS-treated plants produced more flowers and pods, resulting in 55.6 % greater seed yield. ETH treatment significantly increased (P < 0.05) the abscission rate of flowers and pods. Three digital gene expression libraries (STS-, ETH- and control library) were constructed. Illumina sequencing of the three libraries was used to identify 9.7 to 10.5 million unigenes. Strong correlations were observed among different gene expression profiles and specific metabolite groups (such as plant hormone biosynthesis and signal transduction; starch and sucrose metabolism; and secondary metabolism) suggesting the importance of these metabolic pathways during ethylene regulation. Potential ethylene target genes such as 1-aminocyclopropane-1-carboxylate (ACC)-oxidase, 2C protein phosphatases (PP2Cs), MAT1, acetyltransferase, bidirectional sugar transporter SWEET1-like, and so on were identified. These results suggest that ethylene regulates flower and pod abscission in soybean by direct transcriptional regulation of genes that are involved in the metabolic and regulatory processes related to both floral organ development and abscission. Thus, these findings further elucidate the critical role of ethylene in transcriptional regulation of reproductive organ development and abscission.