CHARACTERIZATION OF A NEW VIRUS FROM COCKATOOS WITH PSITTACINE BEAK AND FEATHER DISEASE
CHARACTERIZATION OF A NEW VIRUS FROM COCKATOOS WITH PSITTACINE BEAK AND FEATHER DISEASE
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DOI：
10.1016/0042-6822(89)90513-8
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发表时间：
1989-07-01
期刊：
影响因子：
3.7
通讯作者：
LATIMER, KS
中科院分区：
文献类型：
--
作者：
RITCHIE, BW;NIAGRO, FD;LATIMER, KS
从被诊断患有鹦鹉嘴和羽毛病的凤头鹦鹉的羽毛毛囊中分离出一种新型病毒，并通过电子显微镜、核酸含量和多肽组成对其进行了表征。纯化的病毒颗粒呈现二十面体对称性，无包膜，平均直径为 14 至 16 nm，负染色。鉴定出三种主要病毒蛋白，分子量约为 26.3、23.7 和 15.9 kDa。吖啶橙染色、耐碱和核糖核酸酶、对DNase 1和S1核酸酶敏感，发现病毒核酸为单链DNA。通过琼脂糖凝胶电泳估计 DNA 的大小在 1.7 至 2.0 kb 之间。通过电子显微镜证实了该尺寸及其圆形构象。使用纯化病毒进行的初步传播研究诱导了在自然疾病中观察到的病理损伤特征。基于病毒体的极小尺寸和单链环状病毒DNA，我们提出鹦鹉喙羽病的病原体代表了一种以前未描述过的病毒病原体。
A novel virus isolated from the feather follicles of cockatoos diagnosed as having psittacine beak and feather disease was characterized by electron microscopy, nucleic acid content, and polypeptide composition. Purified virions displayed an icosahedral symmetry, were nonenveloped, and had a mean diameter of 14 to 16 nm negatively stained. Three major viral proteins were identified, with approximate molecular weights of 26.3, 23.7, and 15.9 kDa. The viral nucleic acid was found to be single-stranded DNA based on acridine orange staining, resistance to alkali and ribonuclease, and sensitivity to both DNase 1 and S1 nuclease. The size of the DNA was estimated to be between 1.7 and 2.0 kb by agarose gel electrophoresis. This size and its circular conformation were confirmed by electron microscopy. A preliminary transmission study using purified virus induced pathological lesions characteristic of those observed in the natural disease. On the basis of the extremely small size of the virions and the single-stranded circular viral DNA, we propose that the etiologic agent of psittacine beak and feather disease represents a previously undescribed viral pathogen.