Expression of ERV3-1 in leukocytes of acute myelogenous leukemia patients
Expression of ERV3-1 in leukocytes of acute myelogenous leukemia patients
复制标题
急性髓系白血病患者白细胞中ERV3-1的表达
DOI：
10.1016/j.gene.2020.145363
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发表时间：
2021
期刊：
影响因子：
3.5
通讯作者：
Kotani A.
中科院分区：
文献类型：
--
作者：
Nakagawa S;Kawashima M;Miyatake Y;Kudo K;Kotaki R;Ando K;Kotani A.
急性髓细胞白血病（acute myelogenous leukemia，AML）是主要的血液系统恶性肿瘤之一。在人类基因组中，已经发现有几种来源于逆转录病毒，其中一些与各种癌症的进展有关。因此，为了研究逆转录病毒样基因是否与AML发展相关，我们对150名AML患者和32名健康供体样本的12个逆转录病毒样基因进行了转录组测序分析，其中RNA测序数据来自公共数据库。我们发现内源性逆转录病毒3组成员1的包膜基因ERV 3 -1在本研究中检查的所有AML患者中高表达。特别是，AML患者骨髓系的血液和骨髓细胞比单核细胞AML系的细胞表现出更高的ERV 3 - 1表达。我们还通过免疫组化分析检测了ERV 3 -1的蛋白表达，发现所有12例髓系表型患者和12例单核细胞表型患者中的7例均表达ERV 3 -1蛋白，在某些白血病细胞的膜上观察到特定浓度。转录组分析进一步表明，ERV 3 -1表达上调可能与8号染色体三体相关，因为发现异常在高表达组中比低表达组更常见。然而，这一发现并没有得到免疫组化数据的证实。这种差异可能部分是由于本研究中分析的样本数量较少造成的。虽然确切的相关分子机制尚不清楚，但我们的结果表明ERV 3 -1可能参与AML的发展。
Acute myelogenous leukemia (AML) is one of the major hematological malignancies. In the human genome, several have been found to originate from retroviruses, and some of which are involved in the progression of various cancers. Hence, to investigate whether retroviral-like genes are associated with AML development, we conducted a transcriptome sequencing analysis of 12 retroviral-like genes of 150 AML patients and 32 healthy donor samples, of which RNA sequencing data were obtained from public databases. We found high expression ofERV3-1, an envelope gene of endogenous retrovirus group 3 member 1, in all AML patients examined in this study. In particular, blood and bone marrow cells of the myeloid lineage in AML patients, exhibited higher expression ofERV3-1than those of the monocytic AML lineage. We also examined the protein expression of ERV3-1 by immunohistochemical analysis and found expression of the ERV3-1 protein in all 12 myeloid-phenotype patients and 7 out of 12 monocytic-phenotype patients, with a particular concentration observed at the membrane of some leukemic cells. Transcriptome analysis further suggested that upregulated ERV3-1 expression may be associated with chromosome 8 trisomy as anomaly was found to be more common among the high expression group than the low expression group. However, this finding was not corroborated by the immunohistochemical data. This discrepancy may have been caused, in part, by the small number of samples analyzed in this study. Although the precise associated molecular mechanisms remain unclear, our results suggest that ERV3-1 may be involved in AML development.