GLUTATHIONE PEROXIDASE-ACTIVITY IN SELENIUM-DEFICIENT RAT-LIVER
GLUTATHIONE PEROXIDASE-ACTIVITY IN SELENIUM-DEFICIENT RAT-LIVER
复制标题
DOI：
10.1016/0006-291x(76)90747-6
复制
发表时间：
1976-01-01
影响因子：
3.1
通讯作者：
BURK, RF
中科院分区：
文献类型：
--
作者：
LAWRENCE, RA;BURK, RF
用H2 O2测定时，喂食缺硒饲料2周的大鼠肝脏上清液中的谷胱甘肽过氧化物酶[EC 1.11.1.9]活性为对照组的8%，但用氢过氧化枯烯测定时为对照组的42%。以1.5mM氢过氧化枯烯为底物时，大鼠肝上清的SephadexG-150凝胶过滤色谱中出现两个谷胱甘肽过氧化物酶活性峰。当使用0.25 mM H2 O2作为底物时，仅检测到第1个峰。缺硒大鼠肝脏上清液的色谱图中不存在第1个峰;但在MW = 39，000对应位置洗脱的第2个峰似乎未发生变化。因此，第2峰代表第2谷胱甘肽过氧化物酶活性，其催化有机氢过氧化物的破坏，但对H2 O2几乎没有活性，并且在严重缺硒时持续存在。
Glutathione peroxidase [EC 1.11.1.9] activity in the liver supernatant from rats fed a Se-deficient diet for 2 wk was 8% of control when measured with H2O2 but 42% of control when assayed with cumene hydroperoxide. Two peaks of glutathione peroxidase activity were present in the Sephadex G-150 gel filtration chromatogram of rat liver supernatant when 1.5 mM cumene hydroperoxide was used as substrate. Only the 1st peak was detected when 0.25 mM H2O2 was used as substrate. The 1st peak was absent from chromatograms of Se-deficient rat liver supernatants; but the 2nd peak, which eluted at a position corresponding to MW = 39,000, appeared unchanged. The 2nd peak thus represents a 2nd glutathione peroxidase activity which catalyzes the destruction of organic hydroperoxides but has little activity toward H2O2 and which persists in severe Se deficiency.