BHK CELLS EXPRESSING SINDBIS VIRUS-INDUCED HOMOLOGOUS INTERFERENCE ALLOW THE TRANSLATION OF NONSTRUCTURAL GENES OF SUPERINFECTING VIRUS
BHK CELLS EXPRESSING SINDBIS VIRUS-INDUCED HOMOLOGOUS INTERFERENCE ALLOW THE TRANSLATION OF NONSTRUCTURAL GENES OF SUPERINFECTING VIRUS
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DOI：
10.1128/jvi.54.2.351-357.1985
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
BROWN, DT
中科院分区：
文献类型：
--
作者：
ADAMS, RH;BROWN, DT
利用温度敏感突变体研究了Sindbis病毒排除重复感染同源病毒的过程。在两个RNA阴性互补组中的突变体被发现在不允许的温度下建立干扰的能力存在缺陷。这些突变体无法在混合感染(互补)中建立干扰，这表明两者在共同的基因产物中都存在缺陷。在病毒RNA中编码的非结构基因被附着、穿透和翻译后，同源干扰被发现阻止了超级感染病毒的复制。超感染野生型病毒的非结构基因产物的产生被发现在允许和不允许的温度下促进了某些RNA温度敏感干扰病毒的复制。某些RNA突变体建立同源干扰并在与野生型病毒超输注后表现出促进生长的能力被解释为产生了一个模型，该模型涉及病毒和宿主成分在建立同源干扰和复制Sindbis病毒RNA中的作用。
The process by which Sindbis virus excludes superinfecting homologous virus was investigated with the use of temperature-sensitive mutants. Mutants in 2 RNA-negative complementation groups were found to be defective in their ability to establish interference at the nonpermissive temperature. These mutants were unable to establish interference in a mixed infection (complementation), suggesting that both were defective in a common gene product. Homologous interference was found to block the replication of superinfecting virus after attachment, penetration and translation of the nonstructural genes encoded in the virus RNA. The production of nonstructural gene products of superinfecting wild-type virus was found to enhance the replication of certain RNA- temperature-sensitive interfering viruses at the permissive and the nonpermissive temperature. The ability of certain RNA- mutants to establish homologous interference and to demonstrate enhanced growth after superinfusion with wild-type virus was interpreted to produce a model implicating both virus and host components in the establishment of homologous interference and in the replication of Sindbis virus RNA.