ABP1 is required for organized cell elongation and division in Arabidopsis embryogenesis
ABP1 is required for organized cell elongation and division in Arabidopsis embryogenesis
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DOI：
10.1101/gad.866201
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发表时间：
2001-04-01
影响因子：
10.5
通讯作者：
Jones, AM
中科院分区：
文献类型：
--
作者：
Chen, JG;Ullah, H;Jones, AM
为了直接定位一个被命名为ABP1的生长素受体的功能，采用反向遗传方法来鉴定和鉴定拟南芥中的ABP1突变等位基因。ABP1中的纯合子零突变使胚胎致死。零突变胚胎正常发育，直到球状胚胎的早期阶段，但由于细胞无法伸长，无法过渡到两侧对称的结构。胚柄和胚状体的细胞分裂也发生异常。在烟草细胞中反义抑制ABP1会导致缓慢的增殖，消除生长素诱导的细胞伸长，并减少细胞分裂。单个细胞中完全缺乏生长素诱导的伸长证实了在胚胎中观察到的结果，表明了细胞自主功能，结合ABP1结合生长素的生化证据，表明ABP1介导生长素诱导的细胞伸长，并直接或间接地介导细胞分裂。
To directly address the function of a putative auxin receptor designated ABP1, a reverse genetic approach was taken to identify and characterize ABP1 mutant alleles in Arabidopsis. A homozygous null mutation in ABP1 confers embryo lethality. Null mutant embryos develop normally until the early stages of the globular embryo but are unable to make the transition to a bilaterally symmetrical structure because cells fail to elongate. Cell division was also aberrant both in the suspensor and embryo proper. Antisense suppression of ABP1 in tobacco cells causes slow proliferation and eliminates auxin-induced cell elongation and reduces cell division. The complete lack of auxin-inducible elongation in individual cells confirms the results observed in embryos, indicates a cell autonomous function, and, taken together with biochemical evidence that ABP1 binds auxins, suggests that ABP1 mediates auxin-induced cell elongation and, directly or indirectly, cell division.