Heterogeneity of cycling glial progenitors in the adult mammalian cortex and white matter
Heterogeneity of cycling glial progenitors in the adult mammalian cortex and white matter
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DOI：
10.1002/neu.1043
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发表时间：
2001-08-01
期刊：
影响因子：
--
通讯作者：
Goldman, JE
中科院分区：
文献类型：
--
作者：
Gensert, JM;Goldman, JE
用BrdU标记成年大鼠，从新皮质和皮质下白质分离丰富的周期细胞群，研究成年大鼠脑内有丝分裂活性细胞的异质性和分化潜能。这些人中的大多数都是从标记了O4的大脑区域分离出来的，O4是早期的少突胶质细胞标记物。在组织培养中，这些O4(+)祖细胞获得了成熟少突胶质细胞的糖脂半乳糖脑苷，但不获得星形胶质细胞的中间丝GFAP，少数群体表达中间丝蛋白Vimentin，但不表达O4，培养数天后该群体表达GFAP。第三类周期细胞表达A2B5抗体标记的神经节苷脂，代表皮质下白质中的少数细胞群，但皮质中与O4重叠的主要周期类群之一，也观察到少量的循环NG2(+)细胞。因此，成人大脑中的循环细胞是异质的，其中大多数似乎属于神经胶质细胞系。(C)2001年John Wiley&Sons。Inc.
The heterogeneity and differentiation potential of mitotically active cells in the adult brain were studied by labeling adult rats with BrdU, and isolating an enriched population of cycling cells from neocortex and from subcortical white matter. The majority of this population isolated from either brain region labeled with O4, an early oligodendrocyte marker. In tissue culture, these O4(+) progenitors acquired galactocerebroside, a glycolipid of mature oligodendrocytes, but not GFAP, an intermediate filament of astrocytes, A minority population expressed the intermediate filament protein, vimentin, but not O4, This population expressed GFAP after several days in culture. A third population of cycling cells, expressing the gangliosides labeled with the A2B5 antibody, represented a minority population in subcortical white matter, but one of the major cycling populations in cortex, with substantial overlap with O4, Small populations of cycling NG2(+) cells also were observed. Thus, the cycling cells in the adult brain are heterogeneous, and the majority appear to belong to glial lineages. (C) 2001 John Wiley & sons. Inc.