Cytokine Profile From the Ligamentum Flavum in Patients with Ossification of the Posterior Longitudinal Ligament in the Cervical Spine
Cytokine Profile From the Ligamentum Flavum in Patients with Ossification of the Posterior Longitudinal Ligament in the Cervical Spine
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DOI：
10.1097/brs.0000000000004302
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发表时间：
2022-02-01
期刊：
影响因子：
3
通讯作者：
Imai, Shinji
中科院分区：
文献类型：
--
作者：
Yayama, Takafumi;Mori, Kanji;Imai, Shinji
研究设计。颈椎后纵韧带骨化(OPLL)中细胞因子表达的组织学、免疫组织化学和悬浮阵列分析。目标。这项研究的目的是确定细胞因子谱的变化是否反映了软骨细胞和成骨细胞的成熟与OPLL的发展有关。背景数据摘要。OPLL会在较长的时间内逐渐进展，并可能导致严重的脊髓并发症。然而，治疗方法仅包括针对神经症状的保守治疗或手术减压，而对OPLL的预防性治疗仍然不存在。方法：研究方法。韧带标本取自24例脊椎手术后OPLL患者和5例无OPLL的脊髓型颈椎病/神经根型颈椎病患者。组织切片用于免疫组织化学研究，并从韧带样本中培养原代细胞以进行细胞因子分析。使用悬浮阵列系统，测量27种炎性细胞因子或生长因子的浓度，以产生细胞因子谱。结果。悬浮芯片和免疫印迹分析显示OPLL患者IL-6、IL-1α、碱性成纤维细胞生长因子和RANTES水平显著升高。免疫组织化学分析进一步显示，这些因子存在于韧带基质变性部分的间充质细胞中。结论。我们的发现表明，在骨化过程中细胞因子的特殊变化促进了成骨细胞的分化，从而为OPLL的发病机制提供了新的见解。此外，这项工作支持开发一种新的治疗方法，通过调节细胞因子谱来防止OPLL的进展。
Study Design. Histological, immunohistochemical, and suspension array analyses of cytokine expression in human cervical ossification of the posterior longitudinal ligament (OPLL). Objectives. The aim of this study was to determine whether changes in the cytokine profile reflect the maturation of chondrocytes and osteoblasts are associated with OPLL development. Summary of Background Data. OPLL progresses gradually over a prolonged period and may lead to serious spinal cord complications. However, treatment methods only include conservative therapy for neurological symptoms or surgical decompression, whereas preventive therapy for OPLL remains nonexistent. Methods. Ligamentous samples were harvested from 24 patients with OPLL who underwent spinal surgery, and five control samples from cervical spondylotic myelo/radiculopathy patients without OPLL. Tissue sections were used for immunohistochemical studies and primary cells were cultured from the ligamentous samples for cytokine profiling. Using a suspension array system, concentrations of 27 inflammatory cytokines or growth factors were measured to generate the cytokine profiles. Results. Suspension array and immunoblot analysis revealed significant increments in the levels of interleukin (IL)-6, IL-1 alpha, basic fibroblast growth factor, and RANTES in patients with OPLL. Immunohistochemical analysis further revealed that these factors were present in mesenchymal cells within the degenerative portion of the ligamentous matrix. Conclusion. Our findings suggest that specific changes in the cytokine profile during ossification promote osteoblast differentiation, thereby providing new insights into OPLL pathogenesis. Moreover, this work supports the development of a new therapeutic method for preventing OPLL progression by regulating the cytokine profiles.