Transcriptional profiling of the left and median liver lobes of male F344/N rats following exposure to acetaminophen
Transcriptional profiling of the left and median liver lobes of male F344/N rats following exposure to acetaminophen
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DOI：
10.1080/01926230590522257
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发表时间：
2005-01-01
影响因子：
1.5
通讯作者：
Boorman, GA
中科院分区：
文献类型：
--
作者：
Irwin, RD;Parker, JS;Boorman, GA
肝脏是转录图谱的常见器官，因为它在外源代谢中发挥作用，而且肝脏毒性是对化学物质暴露的常见反应。为了探索不同脑叶取样可能对转录图谱实验的影响，我们检测并比较了五只雄性F344大鼠在毒性和非毒性剂量的扑热息痛暴露下左叶和中叶的基因表达谱。使用微阵列的转录图谱显示，F344大鼠的左肝叶和中叶对低剂量以及导致肝脏毒性的剂量的对乙酰氨基酚的反应明显不同。在左叶和中叶差异表达基因的总数、仅在左叶和中叶唯一差异表达的基因的数量和同一性以及基因表达模式上存在差异。虽然不可能将这些结果推广到扑热息痛或其他大鼠品系以外的化合物，但这些结果突显了采样差异对解释肝脏基因表达谱的潜在影响。
The liver is a common organ for transcriptional profiling because of its role in xenobiotic metabolism and because hepatotoxicity is a common response to chemical exposure. To explore the impact that sampling different lobes may have on transcriptional profiling experiments we have examined and compared gene expression profiles of the left and median lobes of fivers front male F344 rats exposed to toxic and nontoxic doses of acetaminophen. Transcript profiling using microarrays revealed clear differences in the response of the left and median liver lobes of F344 rats to acetaminophen exposure both at low doses as well as doses that caused hepatotoxicity. Differences were found in the total number of differentially expressed genes in the left and median lobes, the number and identity of genes that were differentially expressed uniquely only in the left or median lobe, and in the patterns of gene expression. While it is not possible to generalize these results to compounds other than acetaminophen or other strains of rat, these results highlight the potential impact of sampling differences on the interpretation of gene expression profiles in the liver.