An optimised detector for in-situ high-resolution NMR in microfluidic devices
An optimised detector for in-situ high-resolution NMR in microfluidic devices
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DOI：
10.1016/j.jmr.2015.11.011
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发表时间：
2016-01-01
影响因子：
2.2
通讯作者：
Utz, Marcel
中科院分区：
文献类型：
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作者：
Finch, Graeme;Yilmaz, Ali;Utz, Marcel
由于有限的灵敏度和由磁化率不均匀性引起的谱线展宽，高分辨率核磁共振（NMR）光谱与微流体实验室芯片设备的集成是具有挑战性的。我们提出了一种新的双带状线NMR探头，可容纳平面微流控器件，并获得NMR光谱从一个矩形样品室的芯片上的体积为2 μ l。有限元分析用于联合优化探测器和样本体积几何结构的灵敏度和RF均匀性。优化设计的原型已经建成，其特性已被实验表征。在灵敏度和RF均匀性方面的性能与数值预测密切一致。该系统达到了1.57 nmol根S的检测质量极限，与其他微NMR系统相比非常有利。该芯片/探针系统在7 T磁场下的光谱分辨率优于1.75Hz，具有良好的线形。(C)2015 Elsevier Inc. All rights reserved.
Integration of high-resolution nuclear magnetic resonance (NMR) spectroscopy with microfluidic lab on-a-chip devices is challenging due to limited sensitivity and line broadening caused by magnetic susceptibility inhomogeneities. We present a novel double-stripline NMR probe head that accommodates planar microfluidic devices, and obtains the NMR spectrum from a rectangular sample chamber on the chip with a volume of 2 mu l. Finite element analysis was used to jointly optimise the detector and sample volume geometry for sensitivity and RF homogeneity. A prototype of the optimised design has been built, and its properties have been characterised experimentally. The performance in terms of sensitivity and RF homogeneity closely agrees with the numerical predictions. The system reaches a mass limit of detection of 1.57 nmol root s, comparing very favourably with other micro-NMR systems. The spectral resolution of this chip/probe system is better than 1.75 Hz at a magnetic field of 7 T, with excellent line shape. (C) 2015 Elsevier Inc. All rights reserved.