Metrics of separation performance in chromatography. Part 1. Definitions and application to static analyses.
Metrics of separation performance in chromatography. Part 1. Definitions and application to static analyses.
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色谱分离性能指标。
DOI：
10.1016/j.chroma.2011.06.017
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
L. Blumberg
中科院分区：
文献类型：
--
作者：
L. Blumberg
较早引入的分离性能的度量系统地描述。在提供了适用于各种应用的指标定义后，该研究侧重于静态分析（等温GC，等度LC等）。以及它们的一般分离性能。统计学预期的分离（充分分离）单组分峰数被视为色谱分析一般分离性能的最终指标。这个数字取决于分析的峰值容量和测试混合物中组分的数量。峰容量又取决于色谱柱的分离能力和数据分析系统解析分离较差的峰所需的最低分离度。分离能力是分离测量的更广泛度量的特殊情况，其是柱效率、溶质可分离性和溶质与柱固定相的相互作用水平的函数。导出了静态分析中任意峰对的所有这些度量的理论预测公式。为了更好地了解分离性能的基本度量，定义并找到了用于静态分析的附加度量，例如溶质歧视（溶质速度的相对差异）、分离性利用（每单位分离性的溶质歧视）、特定分离（每单位分离性的分离）等。
Earlier introduced metrics of separation performance are described in a systematic way. After providing the definitions of the metrics suitable for a broad variety of applications, the study focuses on static analyses (isothermal GC, isocratic LC, etc.) and their general separation performance. Statistically expected number of resolved (adequately separated) single-component peaks is treated as the ultimate metric of general separation performance of chromatographic analysis. That number depends on the peak capacity of the analysis and the number of components in a test mixture. The peak capacity, in turn, depends on the separation capacity of a column and the lowest separation required by the data-analysis system for resolving poorly separated peaks. The separation capacity is a special case of a broader metric of the separation measure which is a function of column efficiency, solute separability, and the level of the solute interaction with a column stationary phase. The formulae for theoretical prediction of all these metrics for arbitrary pairs of peaks in static analyses are derived. To provide a better insight into the basic metrics of the separation performance, additional metrics such as the solute discrimination (relative difference in solute velocities), utilization of separability (solute discrimination per unit of their separability), specific separation (the separation per unit of separability), and others are defined and found for static analyses.