Acute BPA exposure-induced oxidative stress, depressed immune genes expression and damage of hepatopancreas in red swamp crayfish Procambarus clarkii
Acute BPA exposure-induced oxidative stress, depressed immune genes expression and damage of hepatopancreas in red swamp crayfish Procambarus clarkii
复制标题
急性BPA暴露诱导克氏原螯虾氧化应激、免疫基因表达抑制和肝胰腺损伤
DOI：
10.1016/j.fsi.2020.04.032
复制
发表时间：
2020
影响因子：
4.7
通讯作者：
Hui Yang
中科院分区：
文献类型：
--
作者：
Yingying Zhang;Kaihang Mi;Wen Xue;Wenzhi Wei;Hui Yang
Bisphenol A is a typical endocrine disrupting chemicals (EDCs) and produce various toxic effects on animals due to its potential endocrine disruption, oxidative damage effect, mutagenic effect and hypomethylation. To study its effect on the immune system of crustaceans, the Procambarus clarkii were utilized to detect the immune related indicators after 225 μg/L BPA exposure for 1 week. Hepatopancreatic histology and ultrastructure analysis showed that the brush border disappeared, the lumen increased, and the connection between the hepatic tubules fade away in BPA treated group. BPA could significantly increase the level of ROS, inhibit the activities of antioxidant-related enzymes (SOD, POD, and CAT), and thereby cause the oxidative stress. The enzyme activities of AKP, ACP and lysozyme in hepatopancreas after BPA exposure were also depressed even after Aeromonas hydrophila infections. The relative expression profiles of immune-related genes after BPA exposure and bacterial infection showed suppressed trends of most selected genes. UnderA. hydrophilainfections, the cumulative mortality of 225 μg/L BPA-treated crayfish was significantly higher than other groups. All these results indicated that BPA exposure had adverse effects on the immune ability ofP. clarkii.The present study will provide an important foundation for further understanding the effects of EDCs on crustacean immune functions.
