Degradation Mechanism of Poly(vinyl alcohol) by Successive Reactions of Secondary Alcohol Oxidase and β-Diketone Hydrolase from Pseudomonas sp.
Degradation Mechanism of Poly(vinyl alcohol) by Successive Reactions of Secondary Alcohol Oxidase and β-Diketone Hydrolase from Pseudomonas sp.
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DOI：
10.1080/00021369.1986.10867494
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发表时间：
1986-04
期刊：
影响因子：
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通讯作者：
K. Sakai;N. Hamada;Yasuto Watanabe
中科院分区：
文献类型：
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作者：
K. Sakai;N. Hamada;Yasuto Watanabe
来自假单胞菌属的仲醇氧化酶催化分子量为220至1500的各种乙烯醇低聚物和β-酮醇如5-羟基-3-庚酮、4-羟基-2-壬酮、3-羟基-5-壬酮、6-羟基-4-壬酮、7-羟基-5-十二酮和8-羟基-6-十三酮的氧化。β-二酮水解酶催化多种脂肪族β-二酮和芳香族β-二酮如1-苯基-1，3-丁二酮和1-苯基-2，4-戊二酮的水解。以聚乙烯醇（PVA）的低分子量模型化合物4，6-壬二醇为原料，用仲醇氧化酶将其氧化成6-羟基-4-壬酮，再用乙醇脱氢酶将其水解成4，6-壬二酮。β-二酮水解酶将4，6-壬二酮水解为2-戊酮和正丁酸。这些反应是化学计量反应，光谱实验证实了仲醇氧化酶氧化PVA中β-二酮结构的存在。氧化聚乙烯醇中β-二酮结构的吸收随氧化程度的增加而降低。
The secondary alcohol oxidase from Pseudomonas sp. catalyzed the oxidation of various vinyl alcohol oligomers with the molecular weight of 220 to 1500 and of β-ketols such as 5-hydroxy-3-heptanone, 4-hydroxy-2-nonanone, 3-hydroxy-5-nonanone, 6-hydroxy-4-nonanone, 7-hydroxy-5-dodecanone, and 8-hydroxy-6-tridecanone. β-Diketone hydrolase from the same strain catalyzed the hydrolysis of various aliphatic β-diketones and some aromatic β-diketones such as 1-phenyl-1,3-butanedione and 1-phenyl-2,4-pentanedione. 4,6-Nonanediol, used as a low molecular weight model of poly(vinyl alcohol) (PVA), was oxidized to 4,6-nonanedione by way of 6-hydroxy-4-nonanone by secondary alcohol oxidase. 4,6-Nonanedione was hydrolyzed to 2-pentanone and n-butyric acid by β-diketone hydrolase. These reactions were stoichiometric.The presence of the β-diketone structure in PVA oxidized by secondary alcohol oxidase was confirmed by spectral experiments. The absorption due to β-diketone structure in the oxidized PVA decreased as it was...