Histologic analysis of liver tissue following hepatic arterial infusion of ferromagnetic particles in a rabbit tumour model
Histologic analysis of liver tissue following hepatic arterial infusion of ferromagnetic particles in a rabbit tumour model
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DOI：
10.1023/a:1022555431476
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发表时间：
2003-09-01
期刊：
影响因子：
3.5
通讯作者：
Gray, BN
中科院分区：
文献类型：
--
作者：
Moroz, P;Metcalf, C;Gray, BN
在小动物模型中，有可能用暴露于交变磁场时产生滞后加热的铁磁颗粒动脉栓塞肝肿瘤。本研究的目的是确定肝组织对动脉灌注铁磁颗粒的反应。八只含有肝VX2癌的兔子接受了悬浮在碘油中的铁磁颗粒的肝动脉输注。60 min后处死4只家兔以确定急性组织反应，14天后处死其他4只家兔以确定长期组织反应。每个受试者的肿瘤，正常肝实质（NHP），肺和胆囊进行了检查，使用光学显微镜，和化学分析铁浓度。肿瘤血管内栓塞的大颗粒聚集体。NHP中仅存在稀疏的颗粒分布，无急性组织反应。肿瘤与NHP铁浓度比为4.9。颗粒也停留在胆囊壁的血管中。输注后2周，NHP中存在孤立的坏死灶，巨噬细胞与颗粒聚集体相关，在多核巨细胞中也观察到颗粒聚集体。没有证据表明颗粒在肺部栓塞。悬浮在碘油中的铁磁颗粒的肝动脉输注导致了极好的肿瘤靶向，在NHP中没有急性组织反应，也没有肺栓塞的证据。14天后，有证据表明NHP中的颗粒被吞噬，但在肿瘤组织中没有。然而，悬浮液在NHP中引起多个梗死灶，可能是由于较大动脉闭塞。
It is possible to arterially embolize liver tumours in small animal models with ferromagnetic particles that generate hysteretic heating on exposure to an alternating magnetic field. The aim of this study was to determine the response of hepatic tissue to arterial infusion of ferromagnetic particles. Eight rabbits containing hepatic VX2 carcinomas received a hepatic arterial infusion of ferromagnetic particles suspended in lipiodol. Four rabbits were sacrificed after 60 min to determine the acute tissue response, and the other four rabbits were sacrificed after 14 days to determine the longer-term tissue response. The tumour, normal hepatic parenchyma (NHP), lungs and gallbladder of each subject were examined using light microscopy, and chemically analysed for iron concentration. Large aggregates of particles embolized within the tumour vasculature. There was only a sparse distribution of particles in the NHP, with no acute tissue response. The tumour to NHP iron concentration ratio was 4.9. Particles also lodged in blood vessels of the gallbladder wall. Two weeks after infusion there were isolated foci of necrosis in the NHP, and macrophages were associated with particle aggregates, which were also observed within multinucleated giant cells. There was no evidence that particles embolized in the lungs. Hepatic arterial infusion of ferromagnetic particles suspended in lipiodol resulted in excellent tumour targeting with no acute tissue reaction in the NHP, and no evidence of pulmonary embolization. After 14 days there was evidence of phagocytosis of the particles in NHP, but not in the tumour tissue. However, the suspension caused multiple foci of infarction in NHP, probably due to occlusion of larger arteries.