Comparative Proteomic Analysis of Aluminum Tolerance in Tibetan Wild and Cultivated Barleys
Comparative Proteomic Analysis of Aluminum Tolerance in Tibetan Wild and Cultivated Barleys
复制标题
西藏野生和栽培大麦铝耐受性的比较蛋白质组分析
DOI：
10.1371/journal.pone.0063428
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发表时间：
2013-05-14
期刊：
影响因子：
3.7
通讯作者：
Wu, Feibo
中科院分区：
文献类型：
--
作者：
Dai, Huaxin;Cao, Fangbin;Wu, Feibo
铝毒性是酸性土壤中植物生产的主要限制因素。野生大麦种质资源具有丰富的遗传多样性，可为作物耐铝性改良提供优良基因。代顿。结果表明，与XZ61和Dayton相比，XZ16在Al胁迫下对Al的吸收和转运较少。鉴定出35种铝耐受性/抗性相关蛋白，并将其分类在代谢、能量、细胞生长/分裂、蛋白质生物合成、蛋白质目的地/储存、转运、信号转导、疾病/防御等方面。其中，30个蛋白在大麦基因组上被定位，16个蛋白在XZ16中被铝胁迫特异性上调，包括4个蛋白（s -腺苷蛋氨酸合成酶3、ATP合成酶β亚基、三磷酸异构酶、Bp2A）在XZ16中特异性表达，而代顿不表达。
Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al- resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.