Determination of human reference values for serum total 1,25-dihydroxyvitamin D using an extensively validated 2D ID-UPLC-MS/MS method
Determination of human reference values for serum total 1,25-dihydroxyvitamin D using an extensively validated 2D ID-UPLC-MS/MS method
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DOI：
10.1016/j.jsbmb.2015.12.003
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发表时间：
2016-11-01
影响因子：
4.1
通讯作者：
Heijboer, Annemieke C.
中科院分区：
文献类型：
--
作者：
Dirks, Niek F.;Martens, Frans;Heijboer, Annemieke C.
背景：为了评估患者的维生素D状态，可以测定前体代谢物25-羟基维生素D。然而，当怀疑1a-羟基化障碍、肾外1a-羟基化障碍或维生素D受体缺陷时，需要测量1，25-二羟基维生素D。方法：本研究的目的是使用2D ID-UPLC-MS/MS方法确定1，25-二羟基维生素D-3和D-2的参考值。LC-MS/MS方法能够测量人血清中1，25-二羟基维生素D3和D2的皮摩尔浓度，并得到广泛验证。试验内变异为
Background: To assess a patient's vitamin D status the precursor metabolite 25-hydroxyvitamin D can be determined. However, measurement of 1,25-dihydroxyvitamin D is required when disorders of 1a-hydroxylation, extrarenal 1a-hydroxylation, or vitamin D receptor defects are suspected.Methods: The aim of this study was to determine reference values for 1,25-dihydroxyvitamin D-3 and D-2 using a 2D ID-UPLC-MS/MS method.Results: The LC-MS/MS method, able to measure picomolar concentrations of both 1,25-dihydroxyvitamin D3 and D2 in human serum, was extensively validated. Intra-assay variations were