Control of cell cycle timing during C. elegans embryogenesis
Control of cell cycle timing during C. elegans embryogenesis
复制标题
DOI：
10.1016/j.ydbio.2008.02.054
复制
发表时间：
2008-06-01
影响因子：
2.7
通讯作者：
Waterston, Robert H.
中科院分区：
文献类型：
--
作者：
Bao, Zhirong;Zhao, Zhongying;Waterston, Robert H.
作为发育的基本过程，细胞增殖必须与其他过程如命运分化相协调。通过对秀丽隐杆线虫胚胎细胞分裂10轮中的前8轮的单个细胞周期长度的统计分析，我们确定了与命运分化密切相关的同步和恒定有序的分裂。我们的研究结果提出了一个三层模型的命运控制细胞周期的步伐：细胞周期的步伐的主要控制建立的谱系和创始人细胞的命运，然后微调组织和器官分化在每个谱系，然后进一步修改个性化的细胞，因为他们获得独特的形态和生理作用的变体身体计划。然后，我们着手确定不同命运的节奏设定机制。我们的研究结果表明，泛素介导的CDC-25.1降解是E（肠道）和P-3（肌肉和生殖系）谱系的速率决定步骤，但不是其他人，即使CDC-25.1及其明显的衰减已在所有谱系中检测到。我们的研究结果表明，作为一个模型，以解剖分化和增殖之间的相互作用，并在单细胞分辨率的遗传和统计分析相结合的有效方法，线虫胚胎发生的权力。(C)2008年爱思唯尔公司All rights reserved.
As a fundamental process of development, cell proliferation must be coordinated with other processes such as fate differentiation. Through statistical analysis of individual cell cycle lengths of the first 8 out of 10 rounds of embryonic cell division in Caenorhabditis elegans, we identified synchronous and invariantly ordered divisions that are tightly associated with fate differentiation. Our results suggest a three-tier model for fate control Of cell cycle pace: the primary Control of cell cycle pace is established by lineage and the founder cell fate, then fine-tuned by tissue and organ differentiation within each lineage, then further modified by individualization of cells as they acquire unique morphological and physiological roles in the variant body plan. We then set out to identify the pace-setting mechanisms in different fates. Our results suggest that ubiquitin-mediated degradation of CDC-25.1 is a rate-determining step for the E (gut) and P-3 (muscle and germline) lineages but not others, even though CDC-25.1 and its apparent decay have been detected in all lineages. Our results demonstrate the power of C elegans embryogenesis as a model to dissect the interaction between differentiation and proliferation, and an effective approach combining genetic and statistical analysis at single-cell resolution. (C) 2008 Elsevier Inc. All rights reserved.