Diversity of Candida zemplinina isolates inferred from RAPD, micro/minisatellite and physiological analysis.
Diversity of Candida zemplinina isolates inferred from RAPD, micro/minisatellite and physiological analysis.
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DOI：
10.1016/j.micres.2013.09.006
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发表时间：
2014-05
影响因子：
6.7
通讯作者：
W. Pfliegler;E. Horváth;Zoltán Kállai;M. Sipiczki
中科院分区：
文献类型：
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作者：
W. Pfliegler;E. Horváth;Zoltán Kállai;M. Sipiczki
在非酿酒酵母中，念珠菌（Candida zemlpininin）是经常被分离出来的重要的酿酒品种之一。它主要来自欧洲酿酒地区，它已成为非酵母菌研究的重点品种之一。调查c的多样性。zemplininaisoles对于深入了解非酵母菌葡萄酒酵母和酵母发酵剂工业非常重要，因为许多研究都指出了该物种在葡萄酒酿造中的潜在用途。为了评估大量菌株的生物多样性，RAPD和微/迷你卫星PCR通常是首选的方法，但这种技术往往不标准化。虽然一些实验室使用这些方法进行物种鉴定，但其他实验室则使用RAPD引物来确定种内多样性。在这项研究中，我们测试了5种不同的RAPD和微/迷你卫星引物对c菌株。zemplinina是从不同地点分离出来的。我们表明，经过严格的pcr优化，旨在重现性和可比性的带模式与这些pcr反应，不同菌株的多样性从广泛的地理位置是相对较低的。对该菌株几个重要的酿酒生理性状的分析也显示出相对较低的多样性水平。我们还证明了c的种内多样性。不同技术（RAPD、微卫星/小卫星或生理分析）的观察结果可能相当不同，不一定具有可比性。
Among non-Saccharomyceswine yeasts,Candida zemlpininais one of the frequently isolated and oenologically important species. It is mostly known from European winemaking areas and it has become one of the key species of non-Saccharomyceswine yeasts to study. Investigating the diversity ofC. zemplininaisolates is important for a deeper understanding of the non-Saccharomyceswine yeasts and for the yeast starter industry, as numerous researches have pointed to the potential use of this species in winemaking. For assessing the biodiversity of a larger number of strains, RAPD and micro/minisatellite PCR is often the method of choice, however, this technique is often unstandardized. Whereas some laboratories use these methods for species identifications, others apply RAPD primers for determining intraspecies diversity. In this study, we have tested 5 different RAPD and micro/minisatellite primers on strains ofC. zemplininaisolated from different locations. We show that after a rigorous PCR-optimization aimed at reproducibility and comparability of band patterns with these PCR-reactions, diversity of different strains from a wide range of geographic locations is relatively low. The analysis of several oenologically important physiological traits of the strains showed a relatively low level of diversity as well. We also demonstrate that the intraspecific diversity ofC. zemplininaobservable with different techniques (RAPD, micro/minisatellite or physiological analysis) may be fairly different and not necessarily comparable.