Tandem mass spectra of tryptic peptides at signal-to-background ratios approaching unity using electrospray ionization high-field asymmetric waveform ion mobility spectrometry/hybrid quadrupole time-of-flight mass spectrometry
Tandem mass spectra of tryptic peptides at signal-to-background ratios approaching unity using electrospray ionization high-field asymmetric waveform ion mobility spectrometry/hybrid quadrupole time-of-flight mass spectrometry
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DOI：
10.1002/rcm.621
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发表时间：
2002-01-01
影响因子：
2
通讯作者：
Guevremont, R
中科院分区：
文献类型：
--
作者：
Barnett, DA;Ding, LY;Guevremont, R
将高场非对称波形离子迁移率谱(FAIMS)与四极飞行时间质谱仪(FAIMS)联用，用于猪血红蛋白胰蛋白肽的串联质谱分析。利用FAIMS，从相对强烈的化学背景中分离出低水平(fmoL/mul)的多电荷胰蛋白酶多肽，使得它们的串联质谱图(MS/MS)缺乏许多使用传统ESI-QqTOFMS仪器观察到的与背景相关的碎片离子。在保持大致相同的绝对强度的同时，实现了一级和串联质谱图的实质性改进。版权所有(C)2002 John Wiley Sons，Ltd.
High-field asymmetric waveform ion mobility spectrometry (FAIMS) has been coupled to a quadrupole time-of-flight mass spectrometer for the tandem mass spectrometric analysis of tryptic peptides of pig hemoglobin. Using FAIMS, low levels (fmol/muL) of multiply charged tryptic peptides were separated from relatively intense chemical background such that their tandem mass spectra (MS/MS) lacked many background-related fragment ions observed using a conventional ESI-QqTOFMS instrument. Substantial improvements in both first-order and tandem mass spectra were realized while maintaining approximately the same absolute intensities. Copyright (C) 2002 John Wiley Sons, Ltd.