Regulation of circGOLPH3 and its binding protein CBX7 on the proliferation and apoptosis of prostate cancer cells.
Regulation of circGOLPH3 and its binding protein CBX7 on the proliferation and apoptosis of prostate cancer cells.
复制标题
DOI：
10.1042/bsr20200936
复制
发表时间：
2020-12-23
影响因子：
4
通讯作者：
Luo S
中科院分区：
文献类型：
--
作者：
Gong L;Tang Y;Jiang L;Tang W;Luo S
为了阐明circGOLPH3对前列腺癌细胞的调控机制，我们在前列腺癌细胞PC-3中进行了过表达和干扰circGOLPH3的实验，然后通过MTT、CCK8、Edu染色、TUNEL染色和流式细胞术评估了前列腺癌细胞的细胞活力、增殖、细胞周期和凋亡。CircGOLPH3的结合蛋白通过RNA拉下、质谱和RNA结合蛋白免疫沉淀（RIP）检测鉴定。采用qRT-PCR检测CircGOLPH3和CBX7的表达。结果表明，过表达circGOLPH3后，pc -3细胞的增殖能力和活力明显提高，凋亡受到抑制。CircGOLPH3可以结合PC-3细胞中高表达的CBX7蛋白。此外，CBX7的功能测试显示，CBX7过表达显著提高了PC-3细胞的增殖能力和活力，减少了细胞凋亡，这与circGOLPH3的作用一致。验证了本研究circGOLPH3及其结合蛋白CBX7能够促进前列腺癌细胞增殖，抑制细胞凋亡。
To clarify the mechanism of circGOLPH3 regulation on prostate cancer cells, we performed an overexpression and interference circGOLPH3 assay in prostate cancer cells PC-3 and then evaluated cellular viability, proliferation, cell cycle, and apoptosis of prostate cancer cells by MTT, CCK8, Edu stain, TUNEL stain, and flow cytometry. Binding proteins of CircGOLPH3 were identified by RNA pull-down, mass spectrometry, and RNA-binding protein immunoprecipitation (RIP) assays. The expressions of CircGOLPH3 and CBX7 were measured by qRT-PCR. The results showed that after overexpression of circGOLPH3, the proliferative capacity and the viability of PC-3cells were significantly improved, whereas apoptosis was inhibited. CircGOLPH3 could bind to the CBX7 protein that was highly expressed in the PC-3 cell. Additionally, a functional test on CBX7 showed that the CBX7 overexpression notably improved the proliferative capacity and the viability of PC-3 cells and decreased cellular apoptosis, which was consistent with the effects of circGOLPH3. The validated the present study that circGOLPH3 and its binding protein CBX7 can promote prostate cancer cell proliferation and inhibit apoptosis.