Diurnal rhythm and stress regulate dendritic architecture and spine density of pyramidal neurons in the rat infralimbic cortex
Diurnal rhythm and stress regulate dendritic architecture and spine density of pyramidal neurons in the rat infralimbic cortex
复制标题
DOI：
10.1016/j.bbr.2009.07.021
复制
发表时间：
2009-12-28
影响因子：
2.7
通讯作者：
Czeh, Boldizsar
中科院分区：
文献类型：
--
作者：
Perez-Cruz, Claudia;Simon, Maria;Czeh, Boldizsar
内侧前额叶皮层（mPFC）参与多种高阶认知功能，并参与应激反应的调节。边缘下皮层（ILC）是mPFC最腹侧的部分，接收来自主昼夜节律起搏器——视交叉上核的强烈传入输入。这一事实提出了一种可能性，与压力类似。昼夜节律可能影响ILC神经元的结构可塑性。在这里，我们研究了昼夜变化结合固定应力是否对ILC III层锥体神经元的树突形态有任何影响。在昼夜周期的两个不同时间点（间隔12小时）采集对照大鼠的前额叶皮质，以及在活动或休息期间暴露于1周日常约束应激的大鼠的前额叶皮质。对高尔基-考克斯染色神经元的树突结构和脊柱密度进行数字化重建和分析。我们发现，对照组大鼠在活动期基底树突总是比静止期更长、更复杂，并且有更多的棘。类似的，尽管不太明显的日差异存在于顶端树突中，但应激影响树突结构的方式是日差异消失或减少。我们的研究结果表明，日节律对ILC中锥体细胞的结构可塑性有独特的影响，应激干扰了这种形式的神经可塑性。(C) 2009 Elsevier B V.版权所有
The medial prefrontal cortex (mPFC) participates in several higher order cognitive functions and is involved in the regulation of the stress response. The infralimbic cortex (ILC), the most ventral part of the mPFC, receives a strong afferent input from the master circadian pacemaker, the suprachiasmatic nucleus. This fact raises the possibility that, similarly to stress. the diurnal rhythm may affect structural plasticity of neurons in the ILC. Here we investigated, whether diurnal changes in combination with immobilization stress have any impact on the dendritic morphology of layer III pyramidal neurons in the ILC. Prefrontal cortices were collected from control rats at two different time points of the diurnal cycle (12 h apart), and from rats exposed to 1-week of daily restraint stress either during their active or resting period. Dendritic architecture and spine density of Golgi-Cox stained neurons were digitally reconstructed and analyzed. We found that in control rats during the active period, the basilar dendrites were always longer and more complex, and had more spines than during the resting period. Similar although less pronounced diurnal differences exist in the apical dendrites Stress affected dendritic architecture in a way that the diurnal differences either disappeared or became reduced in their magnitude Our findings indicate that the diurnal rhythm has a unique impact on the structural plasticity of pyramidal cells in the ILC and that stress interferes with this form of neuroplasticity. (C) 2009 Elsevier B V. All rights reserved.