MONOCYTE CHEMOTACTIC FACTOR PRODUCED BY LARGE VESSEL ENDOTHELIAL-CELLS INVITRO
MONOCYTE CHEMOTACTIC FACTOR PRODUCED BY LARGE VESSEL ENDOTHELIAL-CELLS INVITRO
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DOI：
10.1161/01.atv.6.3.254
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发表时间：
1986-05-01
期刊：
影响因子：
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通讯作者：
FOGELMAN, AM
中科院分区：
文献类型：
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作者：
BERLINER, JA;TERRITO, M;FOGELMAN, AM
培养的兔主动脉和人颈动脉内皮细胞产生一种对单核细胞趋化而对中性粒细胞不趋化的因子。棋盘分析表明，活性是由于趋化性，而不是趋化运动。该因子在含血清和无血清培养基中均可产生。用羧肽酶和胰蛋白酶处理导致趋化活性的抑制，表明该因子是一种肽。兔主动脉、人颈动脉和人脐静脉内皮细胞先前暴露于β迁移极低密度脂蛋白（. β .- vldl）的培养基比未经处理的细胞或暴露于低密度脂蛋白的细胞的培养基具有更强的趋化活性。-单独vldl无趋化活性。我们得出结论，大血管内皮细胞产生单核细胞趋化因子，该因子在细胞暴露于- β - vldl后增加。
Cultured rabbit aortic and human carotid artery endothelial cells produced a factor that was chemotactic for monocytes but not for neutrophils. Checkerboard analysis showed that the activity was due to chemotaxis and not to chemokinesis. The factor was produced in both serum-containing and serumless media. Treatment with carboxypeptidase and trypsin resulted in inhibition of chemotactic activity, indicating that the factor is a peptide. Medium from cultures of rabbit aortic, human carotid artery, and human umbilical vein endothelial cells previously exposed to beta migrating very low density lipoprotein (.beta.-VLDL) had substantially more chemotactic activity than medium from untreated cells or cells exposed to low density lipoprotein. .beta.-VLDL alone had no chemotactic activity. We conclude that large vessel endothelial cells produce a monocyte chemotactic factor that is increased after exposure of the cells to .beta.-VLDL.