DNA double-strand breaks and micronuclei in human blood lymphocytes after repeated whole body exposures to 7T Magnetic Resonance Imaging
DNA double-strand breaks and micronuclei in human blood lymphocytes after repeated whole body exposures to 7T Magnetic Resonance Imaging
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DOI：
10.1016/j.neuroimage.2016.03.023
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发表时间：
2016-06-01
期刊：
影响因子：
5.7
通讯作者：
Speck, Oliver
中科院分区：
文献类型：
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作者：
Fatahi, Mahsa;Reddig, Annika;Speck, Oliver
目的：通过从重复暴露于 7T 磁共振成像 (MRI) 的个体中获得的外周血单核细胞中的脱氧核糖核酸 (DNA) 双链断裂 (DSB) 和微核 (MN) 来评估遗传损伤的程度。材料和方法：该研究方案经当地伦理委员会批准。获得了 22 名健康、不吸烟、不酗酒的男性个体的知情同意书，他们从未接受过放疗/化疗、闪烁扫描，并且在抽血前一年未接受过 X 射线检查。 11 名参与者在使用扫描仪或在扫描仪周围工作时反复接触 7T 和 3T MRI，或经常作为 7T 和低场 MRI 研究对象参与（平均年龄 34 +/- 7 岁）。另一半从未暴露于 7T 或低场 MRI，并作为对照（平均年龄 33 +/- 9 岁）。使用 DNA DSB 的抗伽玛 H2AX 免疫荧光染色和 MN 定量来评估淋巴细胞的损伤。分离的细胞在体外进一步暴露于 7T MRI，无论是单独使用还是在 DNA 损伤药物依托泊苷存在下使用，以确定是否存在任何额外的综合效应。检测DNA损伤修复动力学。结果：反复暴露组与对照组的γ-H2AX病灶/细胞平均碱基水平和MN发生率无显着差异（分别为P=0.618和P=0.535）。细胞在体外额外暴露于 7TMRI 对暴露后 1、20 和 72 小时的 MN 频率和 γ H2AX 病灶没有显着影响。结论：频繁重复的 7T MRI 暴露不会导致可检测到的遗传毒性指数增加和 DNA 修复动力学改变。 (C) 2016 Elsevier Inc. 保留所有权利。
Purpose: To examine the extent of genetic damage, assessed from deoxyribonucleic acid (DNA) double-strand breaks (DSBs) and micronuclei (MN) in peripheral blood mononuclear cells obtained from individuals repeatedly exposed to 7T Magnetic Resonance Imaging (MRI).Materials and methods: The study protocol was approved by the local ethics committee. Informed consent was obtained from 22 healthy, non-smoking, non-alcoholic male individuals, who had never undergone radio-/chemo-therapy, scintigraphy, and had not undergone X-ray examination one year prior blood withdrawal. Eleven participants were repeatedly exposed to 7T and 3T MRI while working with/around scanners or frequently participating as 7T and lower field MRI research subjects (mean age 34 +/- 7 years). The other half was never exposed to 7T or lower field MRI and served as controls (mean age 33 +/- 9 years).The damage in lymphocytes was assessed using anti-gamma H2AX immunofluorescence staining of DNA DSBs and by quantification of MN. Isolated cells were further exposed in vitro to 7T MRI either alone or in the presence of the DNA damaging drug etoposide, to determine if there is any additional combined effect. The kinetics of DNA damage repair were examined.Results: The mean base-level of gamma H2AX foci/cell and incidence of MN between repeatedly exposed and control group were not significantly different (P = 0.618 and P = 0.535, respectively). The additional in vitro exposure of cells to 7TMRI had no significant impact on MN frequencies and gamma H2AX foci at 1, 20 and 72 h after exposure.Conclusion: Frequently repeated 7T MRI exposure did not result in a detectable increase in genotoxicity indices and alterations of DNA repair kinetics. (C) 2016 Elsevier Inc. All rights reserved.