Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium
Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium
复制标题
DOI：
10.1021/pr801024g
复制
发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Stephens, Andrew N.
中科院分区：
文献类型：
--
作者：
Chen, Jenny I-C.;Hannan, Natalie J.;Stephens, Andrew N.
本研究旨在通过二维凝胶电泳（DIGE）鉴定正常月经周期增殖期和分泌期人子宫内膜中差异表达的蛋白。1017个斑点中有196个差异表达（p < 0.05）。质谱鉴定出76种蛋白质，代表41种不同的基因产物。免疫组织化学证实了3种代表性蛋白（Rho-GDI α、CLIC1、PGRMC1）的变化。生物学途径分析发现，Jnk和EGF信号通路是子宫内膜蛋白组分泌中期蛋白表达的关键调控因子。
This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDI alpha, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.