Neural stem cell phenotype of tanycyte-like ependymal cells in the circumventricular organs and central canal of adult mouse brain
Neural stem cell phenotype of tanycyte-like ependymal cells in the circumventricular organs and central canal of adult mouse brain
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DOI：
10.1038/s41598-020-59629-5
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发表时间：
2020-02-18
影响因子：
4.6
通讯作者：
Miyata, Seiji
中科院分区：
文献类型：
--
作者：
Furube, Eriko;Ishii, Haruna;Miyata, Seiji
伸长细胞是室管膜细胞的一个亚型，向脑实质延伸长放射状突起。本研究表明，终末板血管器官、皮质下器官和中央管（CC）血管器官中的细长细胞样室管膜细胞表达神经干细胞（NSC）标记物巢蛋白、胶质纤维酸性蛋白和性别决定区Y.这些细长细胞样室管膜细胞在脑室内连续灌注成纤维细胞生长因子-2和表皮生长因子可促进其增殖。CC中的伸长细胞样室管膜细胞能够形成自我更新的神经球，并主要产生新的星形胶质细胞和少突胶质细胞。胶原酶诱导的小髓质出血增加了CC中伸长细胞样室管膜细胞的增殖。这些结果表明，成年小鼠脑中的伸长细胞样室管膜细胞是NSCs，并提示它们是延髓脑损伤时提供新神经元谱系细胞的来源。
Tanycyte is a subtype of ependymal cells which extend long radial processes to brain parenchyma. The present study showed that tanycyte-like ependymal cells in the organum vasculosum of the lamina terminalis, subfornical organ and central canal (CC) expressed neural stem cell (NSC) marker nestin, glial fibrillar acidic protein and sex determining region Y. Proliferation of these tanycyte-like ependymal cells was promoted by continuous intracerebroventricular infusion of fibroblast growth factor-2 and epidermal growth factor. Tanycytes-like ependymal cells in the CC are able to form self-renewing neurospheres and give rise mostly to new astrocytes and oligodendrocytes. Collagenase-induced small medullary hemorrhage increased proliferation of tanycyte-like ependymal cells in the CC. These results demonstrate that these tanycyte-like ependymal cells of the adult mouse brain are NSCs and suggest that they serve as a source for providing new neuronal lineage cells upon brain damage in the medulla oblongata.