Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype
Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype
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DOI：
10.3389/fmicb.2019.00481
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发表时间：
2019-03-29
影响因子：
5.2
通讯作者：
Vilgalys, Rytas
中科院分区：
文献类型：
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作者：
Bonito, Gregory;Benucci, Gian Maria Niccolo;Vilgalys, Rytas
植物根部相关的微生物共生体包括植物根瘤菌。这些微生物的作用是向宿主提供营养和水，影响植物健康和疾病。植物微生物组是由植物物种、植物基因型、土壤和环境条件决定的，但这些变量的贡献在自然系统中很难相互区分。我们使用生物测定常见花园实验来解耦植物基因型和土壤性质对杨属根瘤菌群落结构的影响。通过 454 焦磷酸测序完成 16S、ITS、28S 和 18S rDNA 的高通量扩增和测序。使用 16S 和 ITS 数据集评估真菌和细菌类群的共关联模式。群落二分真菌-细菌网络和 PERMANOVA 结果将真菌或细菌群落的显着差异归因于土壤起源、土壤化学性质和植物基因型。指示物种分析确定了不同土壤中与杨属相关的一组常见的根部细菌以及内生和外生菌根真菌。然而，没有任何一个分类单元或微生物群表明特定的杨树基因型。真菌-细菌网络在丛枝菌根、内生和外生菌根真菌以及属于根瘤菌目、几丁菌目、噬细胞菌目和伯克霍尔德菌目的细菌中过多。这些结果证明了土壤和植物基因型对地下植物微生物群中真菌-细菌网络的重要性。
Plant root-associated microbial symbionts comprise the plant rhizobiome. These microbes function in provisioning nutrients and water to their hosts, impacting plant health and disease. The plant microbiome is shaped by plant species, plant genotype, soil and environmental conditions, but the contributions of these variables are hard to disentangle from each other in natural systems. We used bioassay common garden experiments to decouple plant genotype and soil property impacts on fungal and bacterial community structure in the Populus rhizobiome. High throughput amplification and sequencing of 16S, ITS, 28S and 18S rDNA was accomplished through 454 pyrosequencing. Co-association patterns of fungal and bacterial taxa were assessed with 16S and ITS datasets. Community bipartite fungal-bacterial networks and PERMANOVA results attribute significant difference in fungal or bacterial communities to soil origin, soil chemical properties and plant genotype. Indicator species analysis identified a common set of root bacteria as well as endophytic and ectomycorrhizal fungi associated with Populus in different soils. However, no single taxon, or consortium of microbes, was indicative of a particular Populus genotype. Fungal-bacterial networks were over-represented in arbuscular mycorrhizal, endophytic, and ectomycorrhizal fungi, as well as bacteria belonging to the orders Rhizobiales, Chitinophagales, Cytophagales, and Burkholderiales. These results demonstrate the importance of soil and plant genotype on fungal-bacterial networks in the belowground plant microbiome.