The relevance and applicability of oocyst prevalence as a read-out for mosquito feeding assays.
The relevance and applicability of oocyst prevalence as a read-out for mosquito feeding assays.
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DOI：
10.1038/srep03418
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发表时间：
2013-12-04
影响因子：
4.6
通讯作者：
Bousema T
中科院分区：
文献类型：
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作者：
Stone WJ;Eldering M;van Gemert GJ;Lanke KH;Grignard L;van de Vegte-Bolmer MG;Siebelink-Stoter R;Graumans W;Roeffen WF;Drakeley CJ;Sauerwein RW;Bousema T
蚊子摄食分析在评估减少疟疾传播的干预措施中很重要。中肠卵囊蚊子的比例通常被用来衡量结果，但在自然低强度感染中，卵囊未破裂对蚊子传染性的影响尚不清楚。通过对破裂和完整卵囊的鉴定，我们发现在低强度恶性疟原虫感染中，66.7-96.7%的感染蚊子在感染后11-21天内发生卵囊破裂，II)卵囊破裂总是导致子孢子释放，III)卵囊破裂导致97.8%的蚊子唾液腺感染，以及IV)1250(IQR 313-2400)唾液腺子孢子在每个破裂卵囊中被发现。这些数据表明，根据低强度感染的卵囊流行率可以合理地预测传染性。检测整个蚊子感染的高通量方法表明，18S PCR而不是环子孢子ELISA法给出了感染后第7天蚊子感染率的可靠近似值。
Mosquito feeding assays are important in evaluations of malaria transmission-reducing interventions. The proportion of mosquitoes with midgut oocysts is commonly used as an outcome measure, but in natural low intensity infections the effect of oocyst non-rupture on mosquito infectivity is unclear. By identifying ruptured as well as intact oocysts, we show that in low intensity P. falciparum infections i) 66.7–96.7% of infected mosquitoes experienced oocyst rupture between 11–21 days post-infection, ii) oocyst rupture led invariably to sporozoite release, iii) oocyst rupture led to salivary gland infections in 97.8% of mosquitoes, and iv) 1250 (IQR 313-2400) salivary gland sporozoites were found per ruptured oocyst. These data show that infectivity can be predicted with reasonable certainty from oocyst prevalence in low intensity infections. High throughput methods for detecting infection in whole mosquitoes showed that 18s PCR but not circumsporozoite ELISA gave a reliable approximation of mosquito infection rates on day 7 post-infection.