Type II collagen-chondroitin sulfate-hyaluronan scaffold cross-linked by genipin for cartilage tissue engineering
Type II collagen-chondroitin sulfate-hyaluronan scaffold cross-linked by genipin for cartilage tissue engineering
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DOI：
10.1016/j.jbiosc.2008.09.020
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发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Chu, I-Ming
中科院分区：
文献类型：
--
作者：
Ko, Chih-Sheng;Huang, Jui-Pin;Chu, I-Ming
由于关节软骨的修复能力有限，因此，开发组织工程化软骨用于关节疾病的治疗是非常必要的。硫酸软骨素（CS）和透明质酸（HA）是软骨细胞外基质（ECM）的组分，并且已知影响软骨细胞的增殖和分化。由IF型胶原、CS和HA组成的支架可以创造一种环境，该环境可以保持细胞的正常表型以促进软骨样结构的再生。在这项调查中，我们准备和特点的三维II型胶原蛋白支架和没有HA和CS。通过冷冻干燥制备的多孔复合支架显示出与京尼平交联后的平均直径为140 - 30 μ m和孔隙率为92-95%的互连孔。体外培养14天后，发现形态学上圆形的软骨细胞均匀地分布在整个海绵中。与无CS和HA的支架相比，CS和HA的支架上聚集蛋白聚糖、II型胶原和软骨寡聚基质蛋白（COMP）的基因表达显著增加。此外，有一个显着更大的积累蛋白聚糖（PG）的支架与CS和HA。(C)2008年，日本生物技术学会。All rights reserved.
Owing to of the limited repair capacity of articular cartilage, it is essential to develop tissue-engineered cartilage for patients suffering from joint disease. Chondroitin sulfate (CS) and hyaluronan (HA) are the components of the cartilage extracellular matrix (ECM) and are known to influence the proliferation and differentiation of chondrocytes. Scaffolds composed of type-if collagen, CS, and HA may create an environment that can preserve the normal phenotype of cells to promote regeneration of cartilage-like constructs. In this investigation, we prepared and characterized 3-dimensional type-II collagen scaffolds both with and without HA and CS. Porous composite scaffolds fabricated by freeze-drying showed interconnected pores with mean diameters of 140 30 pm and porosities of 92-95% after cross-linking with genipin. After a 14-day in vitro culture, morphologically round chondrocytes were found to be uniformly distributed throughout the sponges. Expression of genes of aggrecan, type-II-collagen and cartilage oligomeric matrix protein (COMP) was statistically and significantly increased on scaffolds with CS and HA than those without CS and HA. Furthermore, there was a markedly greater accumulation of proteoglycans (PGs) on the scaffolds with CS and HA. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.