The human T-type amino acid transporter-1: Characterization, gene organization, and chromosomal location
The human T-type amino acid transporter-1: Characterization, gene organization, and chromosomal location
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DOI：
10.1006/geno.2001.6678
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发表时间：
2002-01-01
期刊：
影响因子：
4.4
通讯作者：
Endou, H
中科院分区：
文献类型：
--
作者：
Kim, DK;Kanai, Y;Endou, H
系统T是一个不依赖于Na+的转运系统，选择性地转运芳香氨基酸。在这里，我们确定了人类t型氨基酸转运体-1 (TAT1) cDNA和基因（SLC16A10）的结构。人类TAT1 cDNA编码一个515个氨基酸的蛋白，有12个推测的跨膜结构域。SLC16A10定位于人类6号染色体，定位于6q21-q22。SLC16A10包含6个外显子，长度为136 kb。与大鼠主要存在于肠道的TAT1不同，人的TAT1在人的肾脏和肠道中都有强烈表达。人TAT1在非洲爪蟾卵母细胞中的表达证明了色氨酸、酪氨酸、苯丙氨酸和左旋多巴的转运不依赖于Na+，这表明人TAT1是一种转运子服务系统t。因为人TAT1被认为对肠道和肾脏中芳香氨基酸的有效吸收至关重要，它的缺陷可能与芳香氨基酸转运的中断有关，例如在蓝尿不湿综合征中。
System T is a Na+-independent transport system that selectively transports aromatic amino acids. Here, we determined the structure of the human T-type amino-acid transporter-1 (TAT1) cDNA and gene (SLC16A10). The human TAT1 cDNA encoded a 515-amino-acid protein with 12 putative membrane-spanning domains. Human SLC16A10 was localized on human chromosome 6, mapped to 6q21-q22. SLC16A10 contains six exons spanning 136 kb. In contrast to rat TAT1, which is mainly present in the intestine, human TAT1 was strongly expressed in human kidney as well as in human intestine. Expression of human TAT1 in Xenopus laevis oocytes demonstrated the Na+-independent transport of tryptophan, tyrosine, phenylalanine, and L-dopa, indicating that human TAT1 is a transporter subserving system T. Because human TAT1 is proposed to be crucial to the efficient absorption of aromatic amino acids from intestine and kidney, its defect could be involved in the disruption of aromatic amino-acid transport, such as in blue diaper syndrome.