The EMILIN protein family
The EMILIN protein family
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DOI：
10.1016/s0945-053x(00)00074-3
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发表时间：
2000-08-01
期刊：
影响因子：
6.9
通讯作者：
Spessotto, P
中科院分区：
文献类型：
--
作者：
Colombatti, A;Doliana, E;Spessotto, P
EMILIN是细胞外基质糖蛋白的新家族。这个家族的原型是最初在鸡的提取物中鉴定的鸡EMILIN;然后发现它广泛分布在与弹性蛋白相关的几种组织中，并定位于无定形弹性蛋白和微纤维之间的界面。根据肽段序列，通过RT/PCR方法，从鸡和人的肾脏和心脏cDNA文库中分离出EMILIN基因的cDNA。通过在酵母双杂交系统中使用人EMILIN-1的C-末端片段作为诱饵，还分离出了第二个家族成员EMILIN-2。EMILIN的特征在于C-末端gC 1 q球状结构域、短胶原序列、长卷曲螺旋区和新的富含半胱氨酸的N-末端结构域，其可以被认为是也存在于多聚体蛋白中的家族的标志。EMILIN-1基因定位在染色体2 p23上，与己酮糖激酶基因的启动子区重叠。EMILIN-1的gC 1 q结构域可以形成相对稳定和紧凑的同源三聚体，然后这种缔合之后是二硫键键合的原聚体的多聚体组装。从293细胞的上清液中纯化的重组EMILIN-1代表了几种细胞类型的细胞粘附的非常有效的配体。(C)出版社：Elsevier Science B. V.国际矩阵生物学学会。All rights reserved.
The EMILINs are a new family of glycoproteins of the extracellular matrix. The prototype of this family is the chicken EMILIN that was originally identified in extracts of aortas; it was then found to be widely distributed in several tissues associated with elastin and localized at the interface between amorphous elastin and microfibrils. Based on peptide sequences, chicken and human cDNAs coding for EMILIN were isolated by RT/PCR by screening kidney and heart cDNA libraries. By using a C-terminal fragment of human EMILIN-1 as a bait in the yeast two-hybrid system, a second family member, EMILIN-2, has also been isolated. EMILINs are characterized by a C-terminal gC1q globular domain, a short collagenous sequence, a long coiled-coil region and a new cysteine-rich N-terminal domain that can be considered a hallmark of the family being present also in multimerin. The gene for EMILIN-1 was mapped on chromosome 2p23 overlapping with the promoter region of the ketohexokinase gene. The gC1q domain of EMILIN-1 can form relatively stable and compact homotrimers and this association is then followed by a multimeric assembly of disulfide-bonded protomers. Recombinant EMILIN-1 purified from the supernatant of 293 cells represents a very efficient ligand for cell adhesion of several cell types. (C) 2000 Published by Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.