Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka).
Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka).
复制标题
海参5-HT4受体的鉴定及功能表征
DOI：
10.1038/srep40247
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发表时间：
2017-01-06
影响因子：
4.6
通讯作者：
Wu C
中科院分区：
文献类型：
--
作者：
Wang T;Yang Z;Zhou N;Sun L;Lv Z;Wu C
5-羟色胺（5-HT）是一种重要的神经递质和神经调质，通过5-HT受体（5-HTRs）控制多种感觉和运动功能。5-HT 4 R亚家族与激活腺苷酸环化酶（AC）的Gs蛋白相关，并且参与外周器官中的许多反应。本研究对仿刺参（Apostichopus sp.）5-HT_4 R（Aj 5-HT_4 R）进行了鉴定和表征。克隆的Aj 5-HT 4 R全长cDNA为1，544 bp，开放阅读框为1，011 bp，编码336个氨基酸。生物信息学分析表明该受体属于A类G蛋白偶联受体（GPCR）家族。进一步的实验表明，5-HT能以剂量依赖的方式诱导细胞内cAMP水平的显著升高，并诱导与增强型绿色荧光蛋白融合的Aj 5-HT 4 R（Aj 5-HT 4 R-EGFP）从细胞表面迅速内化到细胞质中。此外，还研究了Aj 5-HT 4 R在夏曲霉中的转录谱。和磷酸果糖激酶（AjPFK）。用实时荧光定量PCR方法对日本血吸虫进行了分析。结果使我们对A中Aj 5-HT 4 R有了基本的了解。为进一步研究该受体的细胞信号转导和调控功能奠定了基础。
Serotonin (5-HT) is an important neurotransmitter and neuromodulator that controls a variety of sensory and motor functions through 5-HT receptors (5-HTRs). The 5-HT4R subfamily is linked to Gs proteins, which activate adenylyl cyclases (ACs), and is involved in many responses in peripheral organs. In this study, the 5-HT4R fromApostichopus japonicus (Aj5-HT4R) was identified and characterised. The cloned full-lengthAj5-HT4R cDNA is 1,544 bp long and contains an open reading frame 1,011 bp in length encoding 336 amino acid proteins. Bioinformatics analysis of theAj5-HT4R protein indicated this receptor was a member of class A G protein coupled receptor (GPCR) family. Further experiments usingAj5-HT4R-transfected HEK293 cells demonstrated that treatment with 5-HT triggered a significant increase in intracellular cAMP level in a dose-dependent manner and induced a rapid internalisation ofAj5-HT4R fused with enhanced green fluorescent protein (Aj5-HT4R-EGFP) from the cell surface into the cytoplasm. In addition, the transcriptional profiles ofAj5-HT4Rin aestivatingA. japonicasand phosphofructokinase (AjPFK) in 5-HT administratedA. japonicushave been analysed by real-time PCR assays. Results have led to a basic understanding ofAj5-HT4R inA. japonicus, and provide a foundation for further exploration of the cell signaling and regulatory functions of this receptor.
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海马中的5-羟色胺受体。
影响因子：
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作者：
Berumen LC;Rodríguez A;Miledi R;García-Alcocer G
通讯作者：
García-Alcocer G
影响因子：
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作者：
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通讯作者：
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通讯作者：
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影响因子：
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作者：
De Maeyer, J. H.;Schuurkes, J. A. J.;Lefebvre, R. A.
通讯作者：
Lefebvre, R. A.
影响因子：
4.3
作者：
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通讯作者：
Sola-Penna, Mauro