Species differences in the infectability of vascular grafts.
Species differences in the infectability of vascular grafts.
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DOI：
10.3109/08941939109140761
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
Symes, J F
中科院分区：
文献类型：
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作者：
Ricci, M A;Mehran, R J;Symes, J F
不同种属对菌血症的易感性可能会影响血管移植物感染的研究结果。本研究比较了犬和猪模型中的人工移植物感染。34只杂种犬和38只约克郡猪接受了用3cm的编织涤纶假体段置换肾下主动脉。在关闭时，每只动物接受10（2）至10（8）个金黄色葡萄球菌（S. aureus)具有良好的抗菌活性。移植物培养1周时在犬中产生了可预测的感染率，而猪仅发生随机感染（犬：23/34;猪：7/38; p = 0.0001）。犬的中位感染剂量（ID 50）为10（2.9），但猪未发生足以确定该值的感染。电子显微镜检查显示，猪移植物的纤维蛋白表面光滑，而犬移植物的纤维蛋白衬里不规则，细菌混入。犬人工血管移植物比猪更易发生血源性感染。由于人类血源性感染是罕见事件，因此猪模型可能更适合代表临床情况。
The susceptibility of different species to bacteremia may influence the results of studies on vascular graft infection. The present study compares prosthetic graft infection in canine and porcine models. Thirty-four mongrel dogs and 38 Yorkshire pigs underwent replacement of the infrarenal aorta with a 3-cm segment of a woven Dacron prosthesis. At the time of closure, each animal received an intravenous inoculum of 10(2) to 10(8) Staphylococcus aureus (S. aureus). Graft cultures at 1 week produced a predictable infection rate in dogs, while pigs developed only random infections (dogs: 23/34; pigs: 7/38; p = .0001). The median infective dose (ID50) in dogs was 10(2.9) but pigs did not develop enough infections to determine this value. Electron microscopy revealed a smooth fibrin surface in grafts explanted from pigs, while grafts from dogs demonstrated bacteria enmeshed in an irregular fibrinous lining. Prosthetic vascular grafts in dogs are more susceptible to hematogenous infection than those in pigs. Because hematogenous infection in humans is a rare event, the swine model may be a more appropriate representation of the clinical situation.