Genital CD8+ T cell response to HIV-1 gag in mice immunized by mucosal routes with a recombinant simian adenovirus
Genital CD8+ T cell response to HIV-1 gag in mice immunized by mucosal routes with a recombinant simian adenovirus
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DOI：
10.1016/j.vaccine.2006.07.011
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发表时间：
2007-01-02
期刊：
影响因子：
5.5
通讯作者：
Pinto, Aguinaldo R.
中科院分区：
文献类型：
--
作者：
de Souza, Ana Paula D.;Haut, Larissa H.;Pinto, Aguinaldo R.
AdC6gag37是一种从黑猩猩腺病毒血清型6衍生的e1缺失腺病毒重组病毒，表达HIV-1密码子优化的gag截断形式，在小鼠鼻内或阴道内免疫后诱导转基因特异性CD8(+) T细胞反应。AdC6gag37在全身和粘膜部位诱导gag特异性CD8(+) T细胞。阴道内或鼻内免疫在生殖道中诱导的gag特异性CD8(+) T细胞的频率通过鼻内启动，然后用相同的载体在阴道内增强而大大增加。此外，用AdC6gag37阴道内免疫可增加生殖道中可检测到的γ δ T细胞的数量。(c) 2006 Elsevier Ltd.版权所有。
AdC6gag37, an E1-deleted adenovirus recombinant derived from the chimpanzee adenovirus serotype 6 expressing a codon-optimized truncated form of gag of HIV-1, was tested for induction of transgene-specific CD8(+) T cell responses upon intranasal or intravaginal immunization of mice. Administration of AdC6gag37 induced gag-specific CD8(+) T cells at systemic and mucosal sites. Frequencies of gag-specific CD8(+) T cells elicited in the genital tract by intravaginal or intranasal immunizations were substantially increased by intranasal priming followed by intravaginal boosting with the same vector. Additionally, intravaginal immunization with AdC6gag37 increased the amount of gamma delta T cells that could be detected in genital tract. (c) 2006 Elsevier Ltd. All rights reserved.