Photoaffinity labeling approaches to elucidate lipid–protein interactions
Photoaffinity labeling approaches to elucidate lipid–protein interactions
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光亲和标记方法阐明脂质与蛋白质相互作用
DOI：
10.1016/j.cbpa.2022.102173
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发表时间：
2022
影响因子：
7.8
通讯作者：
Baskin, Jeremy M.
中科院分区：
文献类型：
--
作者：
Yu, Weizhi;Baskin, Jeremy M.
脂质-蛋白质相互作用是脂质许多不同作用的基础。然而，这些非共价结合事件通常是弱的、瞬时的或依赖于环境线索。光亲和标记可以在天然条件下保留这些相互作用，使其能够进行生物化学分析。通常，光亲和标记探针含有二氮丙环光交联剂和用于富集和下游分析的点击化学手柄。在这篇综述中，我们总结了最近的进展，在理解的二氮丙啶光交联的机制，我们提供了一个概述，最近的应用程序的光亲和标记，以揭示不同类型的脂质与特定成员的蛋白质组的相互作用。
Lipid–protein interactions serve as the basis for many of the diverse roles of lipids. However, these noncovalent binding events are often weak, transient, or dependent upon environmental cues. Photoaffinity labeling can preserve these interactions under native conditions, enabling their biochemical profiling. Typically, photoaffinity labeling probes contain a diazirine photocrosslinker and a click chemistry handle for enrichment and downstream analysis. In this review, we summarize recent advances in the understanding the mechanisms of diazirine photocrosslinking, and we provide an overview of recent applications of photoaffinity labeling to reveal the interactions of diverse types of lipids with specific members of the proteome.