A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond
A novel in vivo model using immunotoxin in the absence of p-glycoprotein to achieve ultra selective depletion of target cells: Applications in trogocytosis and beyond
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DOI：
10.1016/j.jim.2020.112794
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发表时间：
2020-08-01
影响因子：
2.2
通讯作者：
Wong, Wilson
中科院分区：
文献类型：
--
作者：
Brown, Kathryn;Meader, Lucy;Wong, Wilson
要确定特定细胞群体的功能并评估其对体内整体系统的贡献，一种常用的方法是选择性地耗尽该细胞群体并观察其效果。使用单抗将毒素输送到靶细胞可以高度有效地实现这一点。在此，我们描述了一种结合使用免疫毒素和多药耐药(MDR)基因缺陷小鼠的体内模型，以便只有表达靶分子的MDR缺陷细胞会被耗尽，而表达靶分子而MDR充足的细胞则幸免于难。这允许以比以前实现的更高程度的特异性进行有针对性的消耗。我们已经将这项技术应用于研究巨噬细胞增多症，即细胞表面分子的细胞间转移，但这一原理也可以利用已在其他研究领域使用的技术进行调整。
A commonly employed method to determine the function of a particular cell population and to assess its contribution to the overall system in vivo is to selectively deplete that population and observe the effects. Using monoclonal antibodies to deliver toxins to target cells can achieve this with a high degree of efficiency. Here, we describe an in vivo model combining the use of immunotoxins and multidrug resistant (MDR) gene deficient mice so that only MDR deficient cells expressing the target molecule would be depleted while target molecule expressing, but MDR sufficient, cells are spared. This allows targeted depletion at a higher degree of specificity than has been previously achieved. We have applied this technique to study trogocytosis, the intercellular transfer of cell surface molecules, but this principle could also be adapted using technology already available for use in other fields of study.