Transcriptional activity of nuclei in 2-cell blocked mouse embryos
Transcriptional activity of nuclei in 2-cell blocked mouse embryos
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DOI：
10.1016/j.tice.2011.03.005
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发表时间：
2011-08-01
期刊：
影响因子：
2.6
通讯作者：
Bogolyubova, I. O.
中科院分区：
文献类型：
--
作者：
Bogolyubova, I. O.
近交系小鼠的胚胎在2细胞晚期停止分裂，这种现象被称为“体外2细胞阻滞”。在目前的工作中，我们使用5-溴尿苷5 '-三磷酸（BrUTP）的显微注射来检查阻断胚胎的转录状态。我们还追踪了这些胚胎中RNA聚合酶II的过度磷酸化形式的核分布。我们发现，当胚胎处于封闭状态时，转录活性逐渐降低。这伴随着SC 35核斑点大小的显著增加，其开始积累过度磷酸化的RNA聚合酶II。(C)2011爱思唯尔有限公司版权所有。
Embryos of inbred mouse strains stop their cleavage at the late 2-cell stage and this phenomenon is known as the "2-cell block in vitro". In the present work we used microinjections of 5-bromouridine 5'-triphosphate (BrUTP) to examine the transcriptional status of blocked embryos. We also traced nuclear distribution of hyperphosphorylated form of RNA polymerase II in such embryos. We found that transcriptional activity is gradually decreased when the embryos stay in block. This is accompanied by prominent increasing of the size of SC35 nuclear speckles that begin to accumulate hyperphosphorylated RNA polymerase II. (C) 2011 Elsevier Ltd. All rights reserved.