Cytochromes P450-mediated degradation of fuel oxygenates by environmental isolates
Cytochromes P450-mediated degradation of fuel oxygenates by environmental isolates
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DOI：
10.1111/j.1574-6941.2010.00847.x
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发表时间：
2010-05-01
影响因子：
4.2
通讯作者：
Vogel, Timothy M.
中科院分区：
文献类型：
--
作者：
Malandain, Cedric;Fayolle-Guichard, Francoise;Vogel, Timothy M.
研究了红红球菌IFP 2001、佐氏红球菌IFP 2005和戈登氏菌IFP 2009（原分枝杆菌）对燃料含氧化合物[甲基叔丁基醚（MTBE）、乙基叔丁基醚（ETBE）和叔戊基甲基醚（驯服）]的降解作用。从不同环境中分离的比较。菌株IFP 2001、IFP 2005和IFP 2009在ETBE上生长，部分原因是细胞色素P450（CYP 249）的活性。所有这些菌株都能够将ETBE降解为叔丁醇，并携带CYP 249细胞色素P450。他们也能够降解甲基叔丁基醚和驯服，但ETBE降解在所有情况下最有效的，与降解率测定后，ETBE的2.1，3.5和1.6 mmol ETBE g-1干重h-1的菌株IFP 2001，IFP 2005和IFP 2009，分别增长。不同的ethR（编码调节子）和ethB（编码细胞色素P450）基因之间的系统发育关系进行了确定，并显示不同ethB基因之间的高度同源性（> 99%）。通过逆转录酶定量PCR测量，只有ETBE能够诱导菌株IFP 2001和IFP 2005中ethB的表达。我们的研究结果是第一个可能发挥作用的ethB基因在生态ETBE降解的迹象。
The degradation of fuel oxygenates [methyl tert-butyl ether (MTBE), ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME)] by Rhodococcus ruber IFP 2001, Rhodococcus zopfii IFP 2005 and Gordonia sp. IFP 2009 (formerly Mycobacterium sp.) isolated from different environments was compared. Strains IFP 2001, IFP 2005 and IFP 2009 grew on ETBE due in part to the activity of a cytochrome P450, CYP249. All of these strains were able to degrade ETBE to tert-butyl alcohol and are harboring the CYP249 cytochrome P450. They were also able to degrade MTBE and TAME, but ETBE was degraded in all cases most efficiently, with degradation rates measured after growth on ETBE of 2.1, 3.5 and 1.6 mmol ETBE g-1 dry weight h-1 for strains IFP 2001, IFP 2005 and IFP 2009, respectively. The phylogenetic relationships between the different ethR (encoding the regulator) and ethB (encoding the cytochrome P450) genes were determined and showed high identity between different ethB genes (> 99%). Only ETBE was able to induce the expression of ethB in strains IFP 2001 and IFP 2005 as measured by reverse transcriptase quantitative PCR. Our results are a first indication of the possible role played by the ethB gene in the ecology of ETBE degradation.