Osteopontin-deficient bone cells are defective in their ability to produce NO in response to pulsatile fluid flow
Osteopontin-deficient bone cells are defective in their ability to produce NO in response to pulsatile fluid flow
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DOI：
10.1006/bbrc.2001.5780
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发表时间：
2001-10-26
影响因子：
3.1
通讯作者：
Klein-Nulend, J
中科院分区：
文献类型：
--
作者：
Denhardt, DT;Burger, EH;Klein-Nulend, J
骨桥蛋白（OPN）是骨基质的非胶原成分。它介导细胞附着并激活信号转导途径。在这项工作中，用野生型和 OPN-/-（“敲除”）小鼠的长骨碎片培养的骨细胞暴露于脉动流体流 (PFF) 中 60 分钟。定期检测培养基的一氧化氮 (NO) 和前列腺素 E-2 (PGE(2)) 释放。 OPN+/+细胞在PFF开始后5-10分钟表现出NO产生峰值，在15分钟时下降至稳定平台； OPN-/- 细胞产生的 NO 少得多。 PFF 导致两种细胞类型的 PGE2 释放减少，尽管 OPN-/- 细胞在 15-30 分钟窗口内减少较少。 PFF 开始后 60 分钟，两种细胞类型都表现出类似的环氧合酶 2 mRNA 水平增强。这些结果表明，通过增加 NO 和减少 PGE 合成来评估，骨细胞需要 OPN 才能对 PFF 做出充分反应。 (C) 2001 年学术出版社。
Osteopontin (OPN) is a noncollagenous component of bone matrix. It mediates cell attachment and activates signal transduction pathways. In this work, bone cells, cultured from fragments of long bones derived from wild-type and OPN-/- ("knock-out") mice, were exposed to pulsatile fluid flow (PFF) over a 60-min period. The medium was assayed periodically for nitric oxide (NO) and prostaglandin E-2 (PGE(2)) release. OPN+/+ cells exhibited a peak of NO production 5-10 min after the onset of PFF, decreasing to a stable plateau at 15 min; much less NO was produced by the OPN-/- cells. PFF resulted in reduced PGE2 release by both cell types, although the reduction was less for the OPN-/- cells in the 15-30 min window. Both cell types exhibited a similar enhancement of cyclooxygenase2 mRNA levels 60 min after initiation of PFF. These results suggest that bone cells require OPN to respond fully to PFF as assessed by increased NO and reduced PGE synthesis. (C) 2001 Academic Press.