Regulation of cytolytic T-lymphocyte generation by B-cell stimulatory factor
Regulation of cytolytic T-lymphocyte generation by B-cell stimulatory factor
复制标题
B 细胞刺激因子对溶细胞 T 淋巴细胞生成的调节
作者：
M. Widmer;K. Grabstein
溶细胞T淋巴细胞(CTL)的生长和分化受可溶性生长激素的调节，其中白介素2(IL-2)被认为是最重要的生长激素。在此，我们报道了淋巴因子B细胞刺激因子(BSF-1或IL-4)对这些功能活跃的T细胞的产生也有深远的影响。特别是，BSF-1在原代混合白细胞培养(MLC)中是产生CTL的有力辅助因子，并在体外启动的MLC记忆群体中诱导细胞溶解活性。对纯化的重组BSF-1和IL-2的直接比较表明，BSF-1是原发MLC中更有效的CTL辅助因子。有趣的是，这两种淋巴因子的不同之处在于，IL-2，而不是BSF-1，在没有明显抗原刺激的情况下，在未启动的细胞培养中诱导了裂解群体。总而言之，我们的数据提供了迄今为止尚未明确的CTL激活和扩增机制的直接证据。
The growth and differentiation of cytolytic T lymphocytes (CTL) is regulated by soluble growth hormones, of which interleukin-2 (IL-2) is considered to be of prime importance. Here we report that the lymphokine B-cell stimulatory factor (BSF-1 or inter-leukin-4) also has profound effects on the generation of these functionally active T cells. In particular, BSF-1 acts as a potent helper factor for the generation of CTL in primary mixed leukocyte culture (MLC) and induces cytolytic activity in in vitro primed, MLC memory populations. Direct comparison of purified recom-binant BSF–1 and IL-2 reveals BSF-1 to be the more potent CTL helper factor in primary MLC. Interestingly, the two lymphokines differed in that IL-2, but not BSF-1, induced a lytic population in cultures of unprimed cells without an overt antigenic stimulus. Collectively, our data provide a direct demonstration of a heretofore undefined mechanism by which CTL activation and amplification can occur.