Sparse, reliable, and long-term stable representation of periodic whisker deflections in the mouse barrel cortex
Sparse, reliable, and long-term stable representation of periodic whisker deflections in the mouse barrel cortex
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DOI：
10.1016/j.neuroimage.2015.04.045
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发表时间：
2015-07-15
期刊：
影响因子：
5.7
通讯作者：
Weber, Bruno
中科院分区：
文献类型：
--
作者：
Mayrhofer, Johannes M.;Haiss, Florent;Weber, Bruno
啮齿动物须系统是研究体感觉皮层（桶状皮层）可塑性的首选模型。相反，只有少量的研究进行表征神经元群体活动的稳定性在桶皮层。我们使用小鼠须系统来研究体感觉皮层稳定知觉的神经元基础。在麻醉小鼠的长期双光子Ca2+成像中，研究了长时间（长达3个月）在核上层神经元群体中周期性晶须偏转的皮质表征。我们发现，在大多数神经元中（87%），Ca2+反应随着对侧晶须偏转数量的增加而亚线性增加。成像的神经元群在几天内以一种刻板的方式被激活，并以不同的偏转率（脉冲频率）被激活。因此，脉冲频率是由响应强度编码的，而不是由不同的神经元亚群编码的。一小部分高反应神经元（约3%）就足以解码须状刺激。这个保守的功能图谱，由一小群高度反应的神经元领导，可能构成稳定感觉知觉的基础。(C) 2015爱思唯尔公司版权所有。
The rodent whisker system is a preferred model for studying plasticity in the somatosensory cortex (barrel cortex). Contrarily, only a small amount of research has been conducted to characterize the stability of neuronal population activity in the barrel cortex. We used the mouse whisker system to address the neuronal basis of stable perception in the somatosensory cortex. Cortical representation of periodic whisker deflections was studied in populations of neurons in supragranular layers over extended time periods (up to 3 months) with long-term two-photon Ca2+ imaging in anesthetized mice. We found that inmost of the neurons (87%), Ca2+ responses increased sublinearly with increasing number of contralateral whisker deflections. The imaged population of neurons was activated in a stereotypic way over days and for different deflection rates (pulse frequencies). Thus, pulse frequencies are coded by response strength rather than by distinct neuronal sub-populations. A small population of highly responsive neurons (similar to 3%) was sufficient to decode the whisker stimulus. This conserved functional map, led by a small set of highly responsive neurons, might form the foundation of stable sensory percepts. (C) 2015 Elsevier Inc. All rights reserved.