GLYCO PEPTIDES OF THE MEMBRANE GLYCO PROTEIN OF SINDBIS VIRUS
GLYCO PEPTIDES OF THE MEMBRANE GLYCO PROTEIN OF SINDBIS VIRUS
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DOI：
10.1016/0022-2836(70)90314-1
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发表时间：
1970-01-01
影响因子：
5.6
通讯作者：
STRAUSS J H JR
中科院分区：
文献类型：
--
作者：
BURGE B W;STRAUSS J H JR
辛德毕斯病毒（一种虫媒病毒）的膜结构简单，由脂质和一个分子量约为53，000道尔顿的糖蛋白组成，用链霉蛋白酶消化膜糖蛋白可产生一组糖肽（主要是多糖），可通过分子筛进行表征。从鸡成纤维细胞中生长的病毒中，鉴定出三个主要糖肽峰，分子量为3200、2800和1800道尔顿。最小的糖肽，估计含有9个糖残基，仅含有甘露糖和葡糖胺，而较大的糖肽由甘露糖、葡糖胺、半乳糖、岩藻糖和可能的唾液酸组成。每摩尔膜蛋白含有3或4摩尔糖肽。在仓鼠细胞中生长的病毒糖肽的大小分布略有不同，但似乎与鸡生长的辛德毕斯病毒的糖肽非常相似。在感染的细胞中，75%的新合成的糖肽物质存在于膜组分中;这些糖肽的分子量范围与病毒颗粒中的糖肽相同，但平均分子量较小。目前还不可能确定病毒膜糖蛋白的碳水化合物部分的合成是否由病毒酶、宿主酶或两者的某种组合所指定。
The membrane of the Sindbis virus (an arbovirus) is structurally simple; it is composed of lipids and a single glycoprotein of molecular weight estimated at 53,000 daltons.Digestion of the membrane glycoprotein with pronase yields a set of glycopeptides (largely polysaccharide) that can be characterized by molecular sieving. From virus grown in chick fibroblasts, three principal glycopeptide peaks were identified, with molecular weights of 3200, 2800 and 1800 daltons. The smallest glycopeptide, estimated to contain nine sugar residues, contains only mannose and glucosamine, while the larger glycopeptides are composed of mannose, glucosamine, galactose, fucose and, presumably, sialic acid. There are 3 or 4 moles of glycopeptide per mole of membrane protein. Glycopeptides from virus grown in hamster cells have a somewhat different size distribution, but seem grossly similar to the glycopeptides of chick-grown Sindbis virus.In the infected cell, 75% of newly synthesized glycopeptide material is found in a membrane fraction; these glycopeptides are of the same range of molecular weights as those found in virus particles, but are shifted to a smaller average molecular weight. It is not yet possible to determine whether synthesis of the carbohydrate moiety of the virus membrane glycoprotein is specified by viral enzymes, host enzymes or some combination of the two.