Circulating "LncPPARδ" From Monocytes as a Novel Biomarker for Coronary Artery Diseases.
Circulating "LncPPARδ" From Monocytes as a Novel Biomarker for Coronary Artery Diseases.
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DOI：
10.1097/md.0000000000002360
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发表时间：
2016-02
期刊：
影响因子：
1.6
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Cai Y;Yang Y;Chen X;He D;Zhang X;Wen X;Hu J;Fu C;Qiu D;Jose PA;Zeng C;Zhou L
Supplemental Digital Content is available in the text To investigate long noncoding RNA NONHSAT112178 (LncPPARδ) as a biomarker for coronary artery disease (CAD) in peripheral blood monocyte cells, RT-qPCR was performed to validate the microarray results, receiver operating characteristic curve was applied to study the potential of LncPPARδ as a biomarker. Diagnostic models from LncPPARδ alone or combination of risk factors were constructed by Fisher criteria. The expression of genes neighboring the LncPPARδ gene was examined with RT-qPCR in THP-1 cell line treated with LncPPARδ siRNA. Using a diagnostic model by Fisher criteria, the consideration of risk factors increased the optimal sensitivity from 70.00% to 82.00% and decreased the specificity from 94.00% to 78.00%. The consideration of risk factors also increased area under the receiver operating characteristic curve from 0.727 to 0.785 (P = 0.001), from 0.712 to 0.768 (P = 0.01), and from 0.769 to 0.835 (P = 0.07), in the original, training, and test sets, respectively. Finally, we found that the expression of peroxisome proliferator-activated receptor δ (PPARδ), Adipose Differentiation-Related Protein (ADRP), and Angiopoietin-like 4 (ANGPTL4) were affected by LncPPARδ silencing. Our present study indicated that LncPPARδ, especially combined with risk factors, can be a good biomarker for CAD. LncPPARδ regulates the expression of neighboring protein-coding genes, PPARδ and its direct target genes ADRP and ANGPTL4.
