Development and validation of a liquid chromatography-tandem mass spectrometry method for the quantitation of synthetic cannabinoids of the aminoalkylindole type and methanandamide in serum and its application to forensic samples
Development and validation of a liquid chromatography-tandem mass spectrometry method for the quantitation of synthetic cannabinoids of the aminoalkylindole type and methanandamide in serum and its application to forensic samples
复制标题
DOI：
10.1002/jms.1877
复制
发表时间：
2011-02-01
影响因子：
2.3
通讯作者：
Auwaerter, Volker
中科院分区：
文献类型：
--
作者：
Dresen, Sebastian;Kneisel, Stefan;Auwaerter, Volker
在作为“香”或“植物肥料”销售的类似“香料”的草药混合物中发现合成大麻模拟物质后，这些活性化合物已在几个欧洲国家被宣布为受控物质。正如预期的那样，对市场上继续出现的新草药混合物的监测显示，在法律当局采取控制措施后不久，其他化合物就被添加到现有混合物和新产品中。迄今为止，已在草药混合物中检测到几种氨基烷基吲哚类型的化合物，但常用的药物筛选程序仍然无法检测到它们的消耗，这鼓励吸毒者用这些产品替代大麻。警察当局、医院和精神病医生对来自精神病住院患者、急诊室或驾驶健康评估的生物样本中合成大麻素的检测和定量的需求不断增加。因此，根据法医指南，开发了一种液液萃取后的液相色谱-串联质谱方法，用于定量 JWH-015、JWH-018、JWH-073、JWH-081、JWH-200、JWH-250、WIN 55,212-2 和甲烷酰胺，以及检测人血清中的 JWH-019 和 JWH-020。毒理学分析。该方法已成功应用于医院、戒毒治疗中心、法医精神病中心和警察当局提供的 80 名受试者的 101 份血清样本。 57 个样本或 56.4% 被发现至少一种氨基烷基吲哚呈阳性。在任何分析的样品中均未检测到 JWH-019、JWH-020、JWH-200、WIN 55,212-2 和甲烷酰胺。版权所有 (C) 2011 John Wiley & Sons, Ltd.
After the discovery of synthetic cannabimimetic substances in 'Spice'-like herbal mixtures marketed as 'incense' or 'plant fertilizer' the active compounds have been declared as controlled substances in several European countries. As expected, a monitoring of new herbal mixtures which continue to appear on the market revealed that shortly after control measures have been taken by legal authorities, other compounds were added to existing mixtures and to new products. Several compounds of the aminoalkylindole type have been detected so far in herbal mixtures but still their consumption cannot be detected by commonly used drug-screening procedures, encouraging drug users to substitute cannabis with those products. There is a increasing demand on the part of police authorities, hospitals and psychiatrists for detection and quantification of synthetic cannabinoids in biological samples originating from psychiatric inpatients, emergency units or assessment of fitness to drive. Therefore, a liquid chromatography-tandem mass spectrometry method after liquid-liquid extraction for the quantitation of JWH-015, JWH-018, JWH-073, JWH-081, JWH 200, JWH-250, WIN 55,212-2 and methanandamide and the detection of JWH-019 and JWH-020 in human serum has been developed and fully validated according to guidelines for forensic toxicological analyses. The method was successfully applied to 101 serum samples from 80 subjects provided by hospitals, detoxification and therapy centers, forensic psychiatric centers and police authorities. Fifty-seven samples or 56.4% were found positive for at least one aminoalkylindole. JWH-019, JWH-020, JWH-200, WIN 55,212-2 and methanandamide were not detected in any of the analyzed samples. Copyright (C) 2011 John Wiley & Sons, Ltd.