Vaccination of pregnant dams with IntiminO157 protects suckling piglets from Escherichia coli O157:H7 infection
Vaccination of pregnant dams with IntiminO157 protects suckling piglets from Escherichia coli O157:H7 infection
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DOI：
10.1128/iai.70.5.2414-2418.2002
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发表时间：
2002-05-01
影响因子：
3.1
通讯作者：
O'Brien, AD
中科院分区：
文献类型：
--
作者：
Dean-Nystrom, EA;Gansheroff, LJ;O'Brien, AD
牛是肠出血性大肠杆菌（EHEC）O 157：117的重要宿主，可引起人类疾病。奶牛和肉牛均无症状且零星感染肠出血性大肠杆菌。我们的长期目标是开发一种有效的疫苗，以防止牛被感染并将EHEC O 157：H7传播给人类。我们使用新生仔猪被动免疫（作为替代模型），以确定是否针对EHEC O 157粘附素（intimin（O 157））的抗体抑制EHEC定植。将血清抗-intimin滴度小于或等于100的妊娠猪（母猪）用纯化的intimin（O 157）接种两次或用无菌缓冲液假接种。母鼠初乳和血清中的Intimin（O 157）特异性抗体滴度在肠外接种Intimin（O 157）后增加。在接种10（6）CFU滋贺毒素阴性（出于人道原因）EHEC O 157：H7菌株之前，允许新生仔猪接种疫苗或假疫苗的母鼠最长8 h。接种后2 ~ 10天剖检仔猪，采集肠道样品进行细菌学计数测定和组织病理学分析。仔猪摄入初乳含有intimin（O 157）特异性抗体从接种疫苗的母鼠，但不是那些护理假疫苗接种的母鼠，保护EHEC O 157：H7定植和肠道损伤。这些结果确立了intimin（O 157）作为EHEC O 157：117抗传播疫苗的可行候选物。
Cattle are important reservoirs of enterohemorrhagic Escherichia coli (EHEC) O157:117 that cause disease in humans. Both dairy and beef cattle are asymptornatically and sporadically infected with EHEC. Our long-term goal is to develop an effective vaccine to prevent cattle from becoming infected and transmitting EHEC O157:H7 to humans. We used passive immunization of neonatal piglets (as a surrogate model) to determine if antibodies against EHEC O157 adhesin (intimin(O157)) inhibit EHEC colonization. Pregnant swine (dams) with serum anti-intimin titers of less than or equal to100 were vaccinated twice with purified intimin(O157) or sham-vaccinated with sterile buffer. Intimin(O157)-specific antibody titers in colostrum and serum of dams were increased after parenteral vaccination with intimin(O157). Neonatal piglets were allowed to suckle vaccinated or sham-vaccinated dams for up to 8 h before they were inoculated with 10(6) CFU of a Shiga toxin-negative (for humane reasons) strain of EHEC O157:H7. Piglets were necropsied at 2 to 10 days after inoculation, and intestinal samples were collected for determination of bacteriological counts and histopathological analysis. Piglets that ingested colostrum containing intimin(O157)-specific antibodies from vaccinated dams, but not those nursing sham-vaccinated dams, were protected from EHEC O157:H7 colonization and intestinal damage. These results establish intimin(O157) as a viable candidate for an EHEC O157:117 antitransmission vaccine.