Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes.
Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes.
复制标题
负载 GOLPH3 siRNA 的阳离子脂质体抑制神经胶质瘤生长
DOI：
10.1007/s11060-018-2966-6
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Liu H
中科院分区：
文献类型：
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作者：
Yuan Z;Zhao L;Zhang Y;Li S;Pan B;Hua L;Wang Z;Ye C;Lu J;Yu R;Liu H
目的GOLPH 3参与胶质瘤的增殖。本研究旨在证明GOLPH 3可以作为胶质瘤基因治疗的靶点。方法在实验过程中，使用阳离子脂质体与血管肽-2（A2-CL），将siGOLPH 3通过血脑屏障传递到胶质瘤。在体内实验中，利用U87-GFP-Luci-bearing BALB/c小鼠模型，我们证明A2-CL可以特异性地将GOLPH 3-siRNA递送至胶质瘤，并有效地抑制胶质瘤的生长.
PurposeGOLPH3 has been shown to be involved in glioma proliferation. In this study, we aimed to demonstrate that GOLPH3 can serve as a target for glioma gene therapy.MethodsDuring the experiment, cationic liposomes with angiopep-2 (A2-CL) were used to deliver siGOLPH3 crossing the blood–brain barrier and reaching the glioma.ResultsAt the cellular level, the A2-CL/siGOLPH3 could silence GOLPH3 and then effectively inhibited the proliferation of cells. In vivo experiments, using U87-GFP-Luci-bearing BALB/c mouse models, we demonstrated that A2-CL could deliver GOLPH3-siRNA specifically to glioma and effectively inhibit glioma growth.ConclusionsThis study shows that GOLPH3 has great potential as a target for the gene therapy of glioma and is of great value in precise medical applications.