Effects of endothelial cells on proliferation and survival of human mesenchymal stem cells and primary osteoblasts
Effects of endothelial cells on proliferation and survival of human mesenchymal stem cells and primary osteoblasts
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DOI：
10.1002/jor.22130
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发表时间：
2012-10-01
影响因子：
2.8
通讯作者：
Finkenzeller, Guenter
中科院分区：
文献类型：
--
作者：
Steiner, Dominik;Lampert, Florian;Finkenzeller, Guenter
血管生成是骨形成、重塑和再生的基本过程。此外，对于组织工程应用中的骨再生，支持新生血管是必不可少的。这可以通过使用原代内皮细胞进行基于细胞的治疗来实现，原代内皮细胞在植入后能够形成功能血管。在骨复合移植物中，共植入的内皮细胞不仅支持新生血管，而且支持成骨细胞和成骨细胞的成骨分化。在本研究中，我们研究了内皮细胞对人原代成骨细胞(HOBS)和人间充质干细胞(MSCs)增殖和存活的影响。人脐静脉内皮细胞(HUVECs)刺激HOB和MSC增殖，而共培养的HUVECs和MSCs对HUVECs的增殖无影响。HUVEC共培养对HOB和MSC增殖的影响在直接共培养中比在间接共培养中更明显，表明这种作用至少部分依赖于HUVEC与HOBS或MSCs之间异型细胞接触的形成。此外，HUVEC共培养通过增加促凋亡蛋白Bad的磷酸化和失活来减少低血清诱导的HOBS和MSCs的凋亡。综上所述，我们的实验表明，共培养的人脐静脉内皮细胞促进了人脐静脉内皮细胞的增殖，减少了低血清诱导的人脐静脉内皮细胞和骨髓间充质干细胞的凋亡。(C)2012年骨科研究会。由威利期刊出版公司出版，《矫形外科杂志》2012年版30：16821689
Angiogenesis is a fundamental process in bone formation, remodeling, and regeneration. Moreover, for the regeneration of bone in tissue engineering applications, it is essential to support neovascularization. This can be achieved by cell-based therapies using primary endothelial cells, which are able to form functional blood vessels upon implantation. In bone composite grafts, coimplanted endothelial cells do not only support neovascularization but also support osteogenic differentiation of osteoblasts and osteoprogenitor cells. In this study, we investigated the effect of endothelial cells on proliferation and cell survival of human primary osteoblasts (hOBs) and human mesenchymal stem cells (MSCs). Human umbilical vein endothelial cells (HUVECs) stimulated hOB and MSC proliferation, whereas proliferation of HUVECs was unaffected by cocultured hOBs or MSCs. The effect of HUVEC cocultivation on hOB and MSC proliferation was more pronounced in direct cocultures than in indirect cocultures, indicating that this effect is at least partially dependent on the formation of heterotypic cell contacts between HUVECs and hOBs or MSCs. Furthermore, HUVEC cocultivation reduced low-serum induced apotosis of hOBs and MSCs by a mechanism involving increased phosphorylation and inactivation of the proapoptotic protein Bad. In summary, our experiments have shown that cocultured HUVECs increase the proliferation and reduce low-serum induced apoptosis of hOBs and MSCs. (c) 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:16821689, 2012