Matricellular protein Cfl1 regulates cell differentiation.
Matricellular protein Cfl1 regulates cell differentiation.
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DOI：
10.4161/cib.26444
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发表时间：
2013-11-01
影响因子：
--
通讯作者：
Lin X
中科院分区：
文献类型：
--
作者：
Tian X;Lin X
就像组织中的高等真核细胞一样，群落中的微生物细胞对环境刺激的反应是一致的。它们通过基质细胞信号介导的细胞间通讯协调基因表达及其生理和形态状态。粘附蛋白Cfl 1最近被证明是调节真核微生物新生隐球菌的形态发生和生物膜形成的基质细胞信号。Cfl 1在交配集落发育过程中在菌丝亚群中天然高表达。一些Cfl 1蛋白被切割并释放到ECM（细胞外基质）。所释放的外源性Cfl 1激活隐球菌细胞以表达其内源性Cfl 1，进行诱导，并形成结构化的生物膜菌落。在这项研究中，我们证明，N-末端信号肽和新的保守的富含半胱氨酸的SIGC结构域在C-末端的粘附性和这种多功能蛋白的信号活性是至关重要的。真菌基质细胞信号网络的调查，涉及Cfl 1和主调节器的形态发生Znf 2提供了一个基础，以进一步阐明微生物发育中的细胞间通讯。
Like higher eukaryotic cells in tissues, microbial cells in a community act in concert in response to environmental stimuli. They coordinate gene expression and their physiological and morphological states through intercellular communication mediated by matricellular signals. The adhesion protein Cfl1 was recently shown to be a matricellular signal in regulating morphogenesis and biofilm formation in the eukaryotic microbe Cryptococcus neoformans. Cfl1 is naturally highly expressed in the hyphal subpopulation during the mating colony development. Some Cfl1 proteins are cleaved and released to the ECM (extracellular matrix). The released exogenous Cfl1 activates Cryptococcus cells to express their endogenous Cfl1, to undergo filamentation, and to form structured biofilm colonies. In this study, we demonstrate that the N-terminal signal peptide and the novel conserved cysteine-rich SIGC domain at the C-terminus are critical for the adherence property and the signaling activity of this multifunctional protein. The investigation of this fungal matricellular signaling network involving Cfl1 and the master regulator of morphogenesis Znf2 provides a foundation to further elucidate intercellular communication in microbial development.