Molecular characterization of field azoxystrobin-resistant isolates of Botrytis cinerea
Molecular characterization of field azoxystrobin-resistant isolates of Botrytis cinerea
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灰霉病现场抗嘧菌酯分离株的分子特征
DOI：
10.1016/j.pestbp.2008.11.004
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发表时间：
2009-02-01
影响因子：
4.7
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Jiang, Jinhua;Ding, Laisong;Ma, Zhonghua
测定了184个灰葡萄孢（Botrytis cinerea）田间分离物对Qo抑制剂嘧菌酯（azoxystrobin）的敏感性。在184个菌株中，有7个菌株对嘧菌酯表现出抗药性。所有这7个嘧菌酯抗性（AR）菌株也耐苯并咪唑类杀菌剂多菌灵，和二甲酰亚胺类杀菌剂异菌脲。在B的AR菌株中未观察到嘧菌酯与酰胺类杀菌剂啶酰菌胺之间的负交互抗性。灰绿色B细胞色素B（cyt B）基因部分序列的结构分析结果表明，在184个菌株中，有79个菌株在cyt B基因的第143位密码子后插入了一个1204 bp的内含子，所有含有该内含子的菌株对嘧菌酯均敏感。该内含子未从任何AR分离株中检测到。cyt B基因部分序列分析表明，所有AR分离株均在密码子143处发生点突变，导致该密码子位置由甘氨酸变为丙氨酸（G143 A）。基于点突变的原理，建立了一种快速检测B型AR分离株的等位基因特异性PCR方法。灰绿色(C)2008年爱思唯尔公司版权所有© 2016
Sensitivity of 184 Botrytis cinerea field isolates to a Qo inhibitor (QoI) fungicide azoxystrobin was determined in this study. Among the 184 isolates, seven showed resistance to azoxystrobin. All these seven azoxystrobin-resistant (AR) isolates were also resistant to a benzimidazole fungicide carbendazim, and a dicarboximide fungicide iprodione. Negative cross-resistance between azoxystrobin and a carboxamide fungicide boscalid was not observed in AR isolates of B. cinerea. Analysis structure of partial cytochrome b (cyt b) gene from B. cinerea showed that a 1204-bp intron inserted directly after the codon 143 of cyt b gene was detected from 79 out of 184 isolates, and all the isolates having this intron were sensitive to azoxystrobin. This intron was not detected from any AR isolate. Sequence analysis of partial cyt b gene showed that all AR isolates had a point mutation at codon 143, which caused a change of glycine to alanine (G143A) at this codon position. Based on the point mutation, an allele-specific PCR assay was developed for the rapid detection of AR isolates of B. cinerea. (C) 2008 Elsevier Inc. All rights reserved,