Behavioral tests after intracerebral hemorrhage in the rat
Behavioral tests after intracerebral hemorrhage in the rat
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DOI：
10.1161/01.str.0000032302.91894.0f
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发表时间：
2002-10-01
期刊：
影响因子：
8.3
通讯作者：
Xi, GH
中科院分区：
文献类型：
--
作者：
Hua, Y;Schallert, T;Xi, GH
背景和目的：人类脑内出血（ICH）可导致明显的血肿周围水肿形成和神经功能缺损。大鼠脑出血模型，包括向尾状核输注自体血液，已被广泛用于研究水肿形成的机制，但行为结果的检查将改善其临床前效用，并提供更严格的评估随时间推移的病理级联事件。本研究的目的是使用电池的感觉运动功能测试，以检查ICH在大鼠的神经系统的影响，并检查哪些成分的血肿参与产生这些effects. Methods的行为测试所使用的前肢放置，偏好前肢使用的重量转移在垂直探索的圆柱形外壳，和一个转角测试。大鼠进行了测试，从第1天到第28天后，注射自体全血;注射血液加水蛭素（凝血酶抑制剂），包装红细胞，凝血酶，或盐水;或针placementonly.Results-The电池的测试表明，有显着的神经功能缺损，脑出血后第1天，逐步恢复功能超过4周。前肢放置评分随水肿程度的变化而变化。注射凝血酶和注射水蛭素可减轻ICH引起的神经功能缺损。注射浓缩红细胞，这会导致延迟水肿的形成，诱导延迟neurological deficitsConclusions-These测试允许连续监测大鼠脑出血后的神经功能缺损和评估治疗干预。神经功能缺损的时间进程与ICH和注射血液成分的脑水肿的时间进程密切匹配。脑出血后有明显的功能恢复，这可能是适合治疗操作。
Background and Purpose-In humans, intracerebral hemorrhage (ICH) causes marked perihematomal edema formation and neurological deficits. A rat ICH model, involving infusion of autologous blood into the caudate, has been used extensively to study mechanisms of edema formation, but an examination of behavioral outcome would improve its preclinical utility and provide a more rigorous assessment of the pathological cascade of events over time. The purpose of this study was to use a battery of sensorimotor function tests to examine the neurological effects of ICH in the rat and to examine which components of the hematoma are involved in generating those effects.Methods-The behavioral tests used were forelimb placing, preference for forelimb use for weight shifts during vertical exploration of a cylindrical enclosure, and a corner turn test. Rats were tested from day 1 to day 28 after injection of autologous whole blood; injection of blood plus hirudin (thrombin inhibitor), packed red blood cells, thrombin, or saline; or needle placement only.Results-The battery of tests indicated that there were marked neurological deficits by day 1 after ICH, with progressive recovery of function over 4 weeks. The forelimb placing score paralleled changes in edema. Injection of thrombin caused and injection of hirudin reduced the ICH-induced neurological deficits. Injection of packed red blood cells, which causes delayed edema formation, induced delayed neurological deficitsConclusions-These tests allow continuous monitoring of neurological deficits after rat ICH and assessment of therapeutic interventions. The time course of the neurological deficit closely matched the time course of cerebral edema for both ICH and injection of blood components. There was marked recovery of function after ICH, which may be amenable to therapeutic manipulation.