Molecular recognition of enzymes and modulation of enzymatic activity by nanoparticle conformational sensors
Molecular recognition of enzymes and modulation of enzymatic activity by nanoparticle conformational sensors
复制标题
纳米颗粒构象传感器对酶的分子识别和酶活性的调节
DOI：
10.1039/d1cc05699a
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发表时间：
2022
影响因子：
4.9
通讯作者：
Zhao, Yan
中科院分区：
文献类型：
--
作者：
Chen, Kaiqian;Zhao, Yan
酶活性的调节是生物学中动态过程的关键，但很难用合成系统实现。我们在这里报告的N-和C-末端肽溶菌酶的分子印迹纳米粒子具有很强的结合力。酶的结合亲和力与蛋白质结构中肽的构象灵活性相关。值得注意的是，在溶菌酶的C-末端的结合增强了酶在高温下的性能，而在N-末端的结合降低了酶的活性。当这些纳米粒子被点击到磁性纳米粒子上时，也可以用来在一个步骤中从混合物中钓出感兴趣的蛋白质。
Regulation of enzyme activity is key to dynamic processes in biology but is difficult to achieve with synthetic systems. We here report molecularly imprinted nanoparticles with strong binding for the N- and C-terminal peptides on lysozyme. Binding affinity for the enzyme correlated with conformational flexibility of the peptides in the protein structure. Significantly, binding at the C-terminus of lysozyme enhanced the performance of the enzyme at elevated temperatures and that at the N-terminus lowered the enzyme activity. These nanoparticles, when clicked onto magnetic nanoparticles, could also be used to fish out the protein of interest from a mixture in a single step.